Asai Germanium Research Institute Co., Ltd., Suzuranoka, Hakodate, Hokkaido, 042-0958, Japan.
The United Graduate School of Agricultural Science, Iwate University, Ueda, Morioka, Iwate, 020-8550, Japan.
Biol Trace Elem Res. 2018 Jan;181(1):164-172. doi: 10.1007/s12011-017-1020-4. Epub 2017 Apr 20.
Poly-trans-[(2-carboxyethyl)germasesquioxane] (Ge-132) is a water-soluble organogermanium compound that exerts various physiological effects, including anti-inflammatory activity and pain relief. In water, Ge-132 is hydrolyzed to 3-(trihydroxygermyl)propanoic acid (THGP), which in turn is capable of interacting with cis-diol compounds through its trihydroxy group, indicating that this compound could also interact with diol-containing nucleic acid constituents. In this study, we evaluated the ability of THGP to interact with nucleosides or nucleotides via nuclear magnetic resonance (NMR) analysis. In addition, we evaluated the effect of added THGP on the enzymatic activity of adenosine deaminase (ADA) when using adenosine or 2'-deoxyadenosine as a substrate. In solution, THGP indeed formed complexes with nucleotides or nucleosides through their cis-diol group. Moreover, the ability of THGP to form complexes with nucleotides was influenced by the number of phosphate groups present on the ribose moiety. Notably, THGP also inhibited the catalysis of adenosine by ADA in a concentration-dependent manner. Thus, interactions between THGP and important biological nucleic acid constituents might be implicated in the physiological effects of Ge-132.
聚-[（2-羧乙基）锗倍半氧烷]（Ge-132）是一种水溶性有机锗化合物，具有多种生理作用，包括抗炎活性和缓解疼痛。在水中，Ge-132 水解为 3-（三羟基锗基）丙酸（THGP），它可以通过其三羟基与顺式二醇化合物相互作用，表明该化合物也可以与含有二醇的核酸成分相互作用。在这项研究中，我们通过核磁共振（NMR）分析评估了 THGP 与核苷或核苷酸相互作用的能力。此外，我们还评估了当使用腺苷或 2'-脱氧腺苷作为底物时，添加的 THGP 对腺苷脱氨酶（ADA）酶活性的影响。在溶液中，THGP 确实通过其顺式二醇基团与核苷酸或核苷形成复合物。此外，THGP 与核苷酸形成复合物的能力受核糖部分上存在的磷酸基团数量的影响。值得注意的是，THGP 还以浓度依赖的方式抑制 ADA 对腺苷的催化作用。因此，THGP 与重要的生物核酸成分之间的相互作用可能与 Ge-132 的生理作用有关。
