Li Peng-Wei, Zhao Xin, Zhao Yun-Long, Wang Bing-Jian, Song Yang, Shen Zi-Long, Jiang Hong-Jun, Jin Hai, Canavero Sergio, Ren Xiao-Ping
Hand and Microsurgery Center, the Second Affiliated Hospital of Harbin Medical University, Nangang District, Harbin, China.
HEAVEN/GEMINI International Collaborative Group, Turin, Italy.
CNS Neurosci Ther. 2017 Jun;23(6):535-541. doi: 10.1111/cns.12700. Epub 2017 Apr 21.
A successful cephalosomatic anastomosis ("head transplant") requires, among others, the ability to control long-term immune rejection and avoidance of ischemic events during the head transference phase. We developed a bicephalic model of head transplantation to study these aspects.
The thoracic aorta and superior vena cava of a donor rat were anastomosed with the carotid artery and extracorporeal veins of a recipient rat by vascular grafts. Before thoracotomy in the donor rat, the axillary artery and vein of the donor were connected to the carotid and the extracranial vein of the third rat through a silicone tube. The silicone tube was passed through a peristaltic pump to ensure donor brain tissue blood supply. There is no ischemia reperfusion injury in donor brain tissue analyzed by electroencephalogram. Postoperative donor has pain reflex and corneal reflex.
Peristaltic pump application can guarantee the blood supply of donor brain tissue per unit time, while the application of temperature change device to the silicone tube can protect the brain tissue hypothermia, postoperative experimental data show that there is no brain tissue ischemia during the whole operation. The application of vascular grafting can also provide the possibility of long-term survival of the model.
成功的头身吻合术（“头部移植”）尤其需要具备控制长期免疫排斥的能力以及在头部转移阶段避免缺血事件的发生。我们开发了一种头部移植的双头模型来研究这些方面。
通过血管移植物将供体大鼠的胸主动脉和上腔静脉与受体大鼠的颈动脉和体外静脉进行吻合。在供体大鼠开胸之前，供体的腋动脉和静脉通过硅胶管与第三只大鼠的颈动脉和颅外静脉相连。硅胶管穿过蠕动泵以确保供体脑组织的血液供应。通过脑电图分析，供体脑组织未出现缺血再灌注损伤。术后供体有疼痛反射和角膜反射。
应用蠕动泵可保证单位时间内供体脑组织的血液供应，而在硅胶管上应用温度变化装置可保护脑组织低温，术后实验数据表明整个手术过程中无脑组织缺血情况。血管移植的应用也为该模型的长期存活提供了可能性。
