Debode Frédéric, Marien Aline, Gérard Amaury, Francis Frédéric, Fumière Olivier, Berben Gilbert
a Unit Traceability and Authentication , Walloon Agricultural Research Center (CRA-W) , Gembloux , Belgium.
b European Union Reference Laboratory for Animal Proteins in feedingstuffs (EURL-AP) , Gembloux , Belgium.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2017 Aug;34(8):1421-1426. doi: 10.1080/19440049.2017.1320811. Epub 2017 Jul 19.
Insects are rich in proteins and could be an alternative source of proteins to feed animals and humans. Numerous companies have started the production of insects for feed purposes. In Europe, these processed animal proteins are not yet authorised by legislation as many questions still need to be answered concerning this 'novel food'. Authorisations will be possible when methods of authentication of the products are available. In this study we propose real-time PCR methods for the specific detection of the mealworm (Tenebriomolitor), one of the most widely used insects for food and feed production. Two PCR assays are proposed: the first based on the wingless gene and the second based on the cadherin gene. The PCR tests amplify fragments of 87 bp. These qualitative methods were tested according to several performance criteria. The specificity was tested on 34 insect species' DNA, but also on non-insect species including crustacean, mammals, birds and plants. The limit of detection was determined and was below 20 copies for the two PCR tests. The applicability of the tests was demonstrated by the analysis of real-life processed samples containing T. molitor.
昆虫富含蛋白质，可能成为喂养动物和人类的蛋白质替代来源。许多公司已开始生产用于饲料目的的昆虫。在欧洲，这些加工过的动物蛋白尚未得到立法授权，因为关于这种“新型食品”仍有许多问题需要解答。当有产品认证方法时才有可能获得授权。在本研究中，我们提出了用于特异性检测黄粉虫（黄粉虫）的实时PCR方法，黄粉虫是食品和饲料生产中使用最广泛的昆虫之一。提出了两种PCR检测方法：第一种基于无翅基因，第二种基于钙粘蛋白基因。PCR测试扩增87 bp的片段。这些定性方法根据几个性能标准进行了测试。对34种昆虫物种的DNA进行了特异性测试，同时也对包括甲壳类动物、哺乳动物、鸟类和植物在内的非昆虫物种进行了测试。确定了检测限，两种PCR测试的检测限均低于20个拷贝。通过对含有黄粉虫的实际加工样品的分析证明了这些测试的适用性。
