黄粉虫（黄粉虫）的完整线粒体基因组。
Complete mitochondrial genome of yellow meal worm (Tenebrio molitor).
作者信息
Liu Li-Na, Wang Cheng-Ye
机构信息
tate Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China.
Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Research Institute of Resource Insects, Chinese Academy of Forestry, Kunming 650224, China.
出版信息
Dongwuxue Yanjiu. 2014 Nov 18;35(6):537-45. doi: 10.13918/j.issn.2095-8137.2014.6.537.
The yellow meal worm (Tenebrio molitor L.) is an important resource insect typically used as animal feed additive. It is also widely used for biological research. The first complete mitochondrial genome of T. molitor was determined for the first time by long PCR and conserved primer walking approaches. The results showed that the entire mitogenome of T. molitor was 15 785 bp long, with 72.35% A+T content [deposited in GenBank with accession number KF418153]. The gene order and orientation were the same as the most common type suggested as ancestral for insects. Two protein-coding genes used atypical start codons (CTA in ND2 and AAT in COX1), and the remaining 11 protein-coding genes started with a typical insect initiation codon ATN. All tRNAs showed standard clover-leaf structure, except for tRNA(Ser) (AGN), which lacked a dihydrouridine (DHU) arm. The newly added T. molitor mitogenome could provide information for future studies on yellow meal worm.
黄粉虫（黄粉虫属）是一种重要的资源昆虫，通常用作动物饲料添加剂。它也广泛用于生物学研究。首次通过长PCR和保守引物步移法测定了黄粉虫的首个完整线粒体基因组。结果表明，黄粉虫的整个线粒体基因组长度为15785 bp，A+T含量为72.35%[已存入GenBank，登录号为KF418153]。基因顺序和方向与被认为是昆虫祖先的最常见类型相同。两个蛋白质编码基因使用非典型起始密码子（ND2中的CTA和COX1中的AAT），其余11个蛋白质编码基因以典型的昆虫起始密码子ATN开始。除了缺乏二氢尿苷（DHU）臂的tRNA（Ser）（AGN）外，所有tRNA均显示出标准的三叶草叶结构。新添加的黄粉虫线粒体基因组可为今后黄粉虫的研究提供信息。
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