Harmon S A, Johnston J M, Ziegelhoffer T, Richards O C, Summers D F, Ehrenfeld E
Department of Cellular, Viral and Molecular Biology, University of Utah, Salt Lake City.
Virus Res. 1988 May;10(2-3):273-80. doi: 10.1016/0168-1702(88)90022-6.
A cDNA coding for hepatitis A virus (HAV) VP1 and portions of the flanking VP3 and P2 sequences was inserted into the genome of Autographa californica nuclear polyhedrosis virus under the control of the polyhedrin promoter and translational start codon. Cells infected with recombinant virus produced high levels of a 55 kDa protein, identified as containing HAV VP1 by reactivity with anti-VP1 serum. The expressed protein also reacted on immunoblots with human HAV convalescent sera as well as sera from rabbits immunized with intact HAV. This protein was found predominantly in the cytoplasm of infected insect cells, probably as an insoluble aggregate.
编码甲型肝炎病毒（HAV）VP1以及侧翼VP3和P2序列部分的cDNA，在多角体蛋白启动子和翻译起始密码子的控制下，被插入到苜蓿银纹夜蛾核型多角体病毒的基因组中。感染重组病毒的细胞产生了高水平的55 kDa蛋白，通过与抗VP1血清反应鉴定该蛋白含有HAV VP1。表达的蛋白在免疫印迹上也与人HAV恢复期血清以及用完整HAV免疫的兔血清发生反应。该蛋白主要存在于受感染昆虫细胞的细胞质中，可能以不溶性聚集体的形式存在。
