Jiao Ke-Ping, Li Shao-Min, Lv Wen-Yan, Jv Ming-Liang, He Hai-Yan
aDepartment of Internal Neurology, People's Hospital of Gansu Province, Lanzhou bDepartment of Neurology, Shanghai Ninth People's Hospital cDepartment of Psychiatry, Shanghai Key Laboratory of Psychotic Disorders, School of Medicine, Shanghai Mental Health Center, Shanghai Jiao Tong University, Shanghai dDepartment of Rehabilitation and Pain, the First Peoples' Hospital of Yinchuan, Yinchuan eDepartment of Anesthesiology, PLA Hospital of China, Hangzhou, China.
Neuroreport. 2017 Jun 14;28(9):492-497. doi: 10.1097/WNR.0000000000000782.
Vitamin D3 has been reported to be an immunity modulator and high levels of vitamin D3 are correlated with a decreased risk for developing diseases in the central nervous system. Astrocytes are important immune cells and contribute toward inflammation during neurological diseases. The vitamin D receptor has been reported to be expressed in astrocytes; however, the effect of vitamin D3 on astrocyte activation has not been studied. Here, we found that lipopolysaccharide stimulation in astrocytes could enhance the expression of vitamin D receptor and Cyp27B1, which encodes the enzyme for converting vitamin D3 into its active form. Vitamin D3 suppressed the expression of proinflammatory cytokines tumour necrosis factor-α, interleukin-1β, vascular endothelial growth factor, and also TLR4 in activated astrocytes. Astrocyte activation was further found to be suppressed after the administration of vitamin D3 in neonatal rats injected with lipopolysaccharide in vivo. We demonstrated the antiactivation effect of vitamin D3 in astrocytes after lipopolysaccharide stimulation. Considering the function of reactive astrocytes in augmenting inflammatory response in neurodegeneration and brain injury, the finding that vitamin D3 administration may inhibit astrocyte activation may be potentially useful for the treatment of central nervous system disorders.
据报道，维生素D3是一种免疫调节剂，高水平的维生素D3与中枢神经系统疾病发病风险降低相关。星形胶质细胞是重要的免疫细胞，在神经疾病期间会引发炎症。据报道，维生素D受体在星形胶质细胞中表达；然而，维生素D3对星形胶质细胞激活的影响尚未得到研究。在此，我们发现星形胶质细胞中的脂多糖刺激可增强维生素D受体和Cyp27B1的表达，Cyp27B1编码将维生素D3转化为其活性形式的酶。维生素D3可抑制活化星形胶质细胞中促炎细胞因子肿瘤坏死因子-α、白细胞介素-1β、血管内皮生长因子以及TLR4的表达。进一步发现，在体内注射脂多糖的新生大鼠中给予维生素D3后，星形胶质细胞的激活受到抑制。我们证明了脂多糖刺激后维生素D3对星形胶质细胞具有抗激活作用。考虑到反应性星形胶质细胞在神经退行性变和脑损伤中增强炎症反应的功能，维生素D3给药可能抑制星形胶质细胞激活这一发现可能对中枢神经系统疾病的治疗具有潜在用途。
