Marshall B, Flynn P, Kamely D, Levy S B
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111.
Appl Environ Microbiol. 1988 Jul;54(7):1776-83. doi: 10.1128/aem.54.7.1776-1783.1988.
The survival of a laboratory strain and a naturally occurring fecal strain of Escherichia coli, with and without a Tn5-containing derivative of ColE1, was examined after aerosol dispersal in a laboratory office and a barn under ambient temperature and humidity conditions. Following the release of paired strains, air and diverse types of surfaces were assayed for the test organisms. In both environments, the number of airborne bacteria declined rapidly within the first 2 h. Longer survival was found on surfaces and varied with surface type: recovery was greatest from wood products. Organisms persisted for 1 day in the office and for up to 20 days in the barn. Survival of the fecal strain was better than that of the laboratory strain in both test environments. In general, plasmid-bearing strains fared similarly to their plasmidless parents, but in several comparisons the ColE1::Tn5-containing strain showed enhanced survival. These studies have implications for the present and proposed release of genetically engineered organisms with and without plasmid vectors.
在环境温度和湿度条件下，将含ColE1的Tn5衍生物和不含该衍生物的大肠杆菌实验室菌株及天然粪便菌株通过气溶胶形式散布于实验室办公室和谷仓后，检测了它们的存活情况。释放配对菌株后，对空气和各种类型的表面进行检测，以确定测试微生物的存在。在这两种环境中，空气中的细菌数量在最初2小时内迅速下降。在表面上发现存活时间更长，且因表面类型而异：从木制品上回收的细菌数量最多。这些微生物在办公室中可存活1天，在谷仓中可存活长达20天。在两种测试环境中，粪便菌株的存活情况均优于实验室菌株。总体而言，携带质粒的菌株与其无质粒亲本的存活情况相似，但在几次比较中，含ColE1::Tn5的菌株显示出更高的存活率。这些研究对目前以及提议的携带和不携带质粒载体的基因工程生物的释放具有启示意义。
