Stojiljković I, Trgovcević Z, Salaj-Smic E
Ruder Bosković Institute, Zagreb, Croatia Yugoslavia.
Gene. 1991 Mar 1;99(1):101-4. doi: 10.1016/0378-1119(91)90039-e.
The rpsL gene of Escherichia coli was inserted into the BamHI site of transposon Tn5. This transposon was called Tn5-rpsL. Tn5-rpsL may be useful in microbiological studies when one wants to cure various bacterial genera of certain plasmid(s). A streptomycin-resistant (SmR) derivative of the host bacterial strain is first isolated. The plasmid(s) later to be cured are then labelled with Tn5-rpsL, which makes the cells Sm-sensitive. These cells can regain their resistance to Sm if they lose the Tn5-rpsL-tagged plasmid. Thus, plasmid-free bacteria are easily selected among SmR survivors. The frequency of occurrence of the plasmid-less variants of plasmid-containing wild-type Salmonella typhimurium measured by this method is given as an example.
将大肠杆菌的rpsL基因插入转座子Tn5的BamHI位点。这个转座子被称为Tn5-rpsL。当人们想要去除某些细菌属中的特定质粒时，Tn5-rpsL在微生物学研究中可能会有用。首先分离出宿主细菌菌株的链霉素抗性（SmR）衍生物。然后用Tn5-rpsL标记随后要去除的质粒，这会使细胞对链霉素敏感。如果这些细胞失去了带有Tn5-rpsL标记的质粒，它们就能恢复对链霉素的抗性。因此，在链霉素抗性幸存者中很容易筛选出无质粒细菌。以这种方法测定的含质粒野生型鼠伤寒沙门氏菌无质粒变体的出现频率为例给出。
