Zhang Yuxiao, Zheng Lulu, Tuo Jiang, Liu Qi, Zhang Xinlian, Xu Zhixuan, Liu Sixiu, Sui Guodong
Shanghai Key Laboratory of Atmospheric Particle Pollution Prevention (LAP3), Department of Environmental Science & Engineering, Fudan University, 220 Handan Road, Shanghai 200433, PR China.
Department of Dermatology, Huashan Hospital, Fudan University, 12 Middle Urumqi Road, Shanghai 200040, PR China.
Toxicol In Vitro. 2017 Sep;43:1-8. doi: 10.1016/j.tiv.2017.04.018. Epub 2017 Apr 18.
Human exposure to PM causes several adverse health effects. Skin is the first barrier against harmful environmental substances and can directly contact with PM, but there is no study about PM-induced cytotoxicity in human skin cells on the molecular level partially due to the shortcomings of traditional research methods. In present study, we established a microfluidic system including a cell culture chip integrated with a high-throughput protein microarray chip to investigate the mechanism of PM-mediated cytotoxicity in human HaCaT cells. We found that PM was lodged inside the cytoplasm, mitochondria and nucleus of HaCaT cells by TEM. Flow cytometry analysis indicated that the cell apoptosis rate increased from 0.49% to 53.4%. The results of protein microarray showed that NF-κB and NALP3 signal transductions were activated in HaCaT cells after PM stimulations, up-regulating the expression of IL-1β and IL-6, which resulted in inflammatory response in HaCaT cells. Our findings provide a molecular insight into PM-induced skin injury.
人类接触细颗粒物会导致多种不良健康影响。皮肤是抵御有害环境物质的第一道屏障，可直接接触细颗粒物，但由于传统研究方法的局限性，目前尚无关于细颗粒物在分子水平上对人皮肤细胞产生细胞毒性的研究。在本研究中，我们建立了一个微流控系统，该系统包括一个与高通量蛋白质微阵列芯片集成的细胞培养芯片，以研究细颗粒物介导的人HaCaT细胞细胞毒性机制。通过透射电子显微镜我们发现细颗粒物沉积在HaCaT细胞的细胞质、线粒体和细胞核内。流式细胞术分析表明细胞凋亡率从0.49%增加到53.4%。蛋白质微阵列结果显示，细颗粒物刺激后，HaCaT细胞中的NF-κB和NALP3信号转导被激活，上调了IL-1β和IL-6的表达，从而导致HaCaT细胞发生炎症反应。我们的研究结果为细颗粒物诱导的皮肤损伤提供了分子层面的见解。
