Kwack Mi Hee, Ha Nam Gyoung, Lee Weon Ju
Department of Immunology and BK21 FOUR KNU Convergence Educational Program of Biomedical Sciences for Creative Future Talents, School of Medicine, Kyungpook National University, Daegu, Korea.
Department of Dermatology, School of Medicine, Kyungpook National University, Daegu, Korea.
Ann Dermatol. 2022 Jun;34(3):163-172. doi: 10.5021/ad.2022.34.3.163. Epub 2022 May 20.
Particulate matter (PM) is one of the air pollutants that can damage human skin; the recent increase in the amount of PM may be detrimental to skin health.
We aimed to investigate the effects of PM on cultured human sebocytes and outer root sheath (ORS) cells and the effects of Siegesbeckia Herba extract (SHE) on PM-treated cultured cells.
Sebocytes and ORS cells were cultured. The cultured cells were treated with various concentrations of PM of <10 µm in size (PM10) (10 µg/ml, 25 µg/ml, 50 µg/ml, and 100 µg/ml) for 24 h. Real-time polymerase chain reaction, measurement of reactive oxygen species (ROS), small interfering (si) RNA transfection, Oil Red O and Nile red staining, and immunofluorescence staining were performed to analyze the presence of inflammatory cytokines, matrix metalloproteinases (MMPs), aryl hydrocarbon receptor (AhR), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), ROS, and lipid production. In addition, PM10 (100 µg/ml)-treated cultured cells were treated with 10 mg/ml of SHE.
PM10 upregulates the expression of inflammatory cytokines, MMPs, AhR, NF-κB, and ROS in cultured human sebocytes and ORS cells. The production of ROS was dramatically reduced in AhR siRNA-transfected cells. In addition, PM10 upregulates sebum production in cultured sebocytes. SHE inhibited the upregulation of inflammatory cytokines, MMPs, AhR, NF-κB, ROS, and sebum production in cultured human sebocytes and/or ORS cells by PM10.
Effects of PM10 on cultured human sebocytes and ORS cells can be regulated by SH.
颗粒物（PM）是可损害人体皮肤的空气污染物之一；近期PM量的增加可能对皮肤健康有害。
我们旨在研究PM对培养的人皮脂腺细胞和外根鞘（ORS）细胞的影响，以及豨莶草提取物（SHE）对经PM处理的培养细胞的影响。
培养皮脂腺细胞和ORS细胞。将培养的细胞用不同浓度的粒径<10 µm的PM（PM10）（10 µg/ml、25 µg/ml、50 µg/ml和100 µg/ml）处理24小时。进行实时聚合酶链反应、活性氧（ROS）测量、小干扰（si）RNA转染、油红O和尼罗红染色以及免疫荧光染色，以分析炎性细胞因子、基质金属蛋白酶（MMP）、芳烃受体（AhR）、活化B细胞核因子κB（NF-κB）、ROS和脂质生成的存在情况。此外，用10 mg/ml的SHE处理经PM10（100 µg/ml）处理的培养细胞。
PM10上调培养的人皮脂腺细胞和ORS细胞中炎性细胞因子、MMP、AhR、NF-κB和ROS的表达。在经AhR siRNA转染的细胞中，ROS的生成显著减少。此外，PM10上调培养的皮脂腺细胞中的皮脂生成。SHE抑制PM10对培养的人皮脂腺细胞和/或ORS细胞中炎性细胞因子、MMP、AhR、NF-κB、ROS和皮脂生成的上调作用。
SH可调节PM10对培养的人皮脂腺细胞和ORS细胞的影响。
