Shahzad Huma, Giribabu Nelli, Karim Kamarulzaman, Kassim Normadiah, Muniandy Sekaran, Kumar Kilari Eswar, Salleh Naguib
Department of Physiology, Faculty of Medicine, University of Malaya, 50603 Lembah Pantai, Kuala Lumpur, Malaysia.
Department of Anatomy, Faculty of Medicine, University of Malaya, 50603 Lembah Pantai, Kuala Lumpur, Malaysia.
Reprod Toxicol. 2017 Aug;71:42-54. doi: 10.1016/j.reprotox.2017.04.004. Epub 2017 Apr 18.
Quercetin could induce changes to the fluid volume and receptivity development of the uterus during peri-implantation period.
Female rats were treated with quercetin (10, 25 and 50mg/kg/day) subcutaneously beginning from day-1 pregnancy. Uterus was harvested at day-4 (following three days quercetin treatment) for morphological, ultra-structural, protein and mRNA expressional changes and plasma sex-steroid levels analyses. In another cohort of rats, implantation rate was determined at day-6 (following five days quercetin treatment).
Administration of 50mg/kg/day quercetin causes increased in uterine fluid volume and CFTR expression but decreased in γ-ENaC, AQP-5, AQP-9 claudin-4, occludin, E-cadherin, integrin αnβЗ, FGF, Ihh and Msx-1expression in the uterus. Pinopodes were poorly develop, tight junctions appear less complex and implantation rate decreased. Serum estradiol levels increased but serum progesterone levels decreased.
Interference in the fluid volume and receptivity development of the uterus during peri-implantation period by quercetin could adversely affect embryo implantation.
槲皮素可能在植入前期引起子宫液体积和接受性发育的变化。
从妊娠第1天开始，对雌性大鼠皮下注射槲皮素（10、25和50mg/kg/天）。在第4天（槲皮素治疗三天后）采集子宫，进行形态学、超微结构、蛋白质和mRNA表达变化以及血浆性甾体水平分析。在另一组大鼠中，在第6天（槲皮素治疗五天后）测定着床率。
每天给予50mg/kg槲皮素会导致子宫液体积增加和CFTR表达增加，但子宫中γ-ENaC、AQP-5、AQP-9、claudin-4、occludin、E-钙黏蛋白、整合素αnβЗ、FGF、Ihh和Msx-1表达减少。微绒毛发育不良，紧密连接看起来不那么复杂，着床率降低。血清雌二醇水平升高但血清孕酮水平降低。
槲皮素在植入前期对子宫液体积和接受性发育的干扰可能会对胚胎着床产生不利影响。
