Xu Lili, Cheng Xianglong, Wu Shuangxiu, Wang Quanxi
Department of Biology, College of Life and Environmental Science, Shanghai Normal University, Guilin Road 100, Shanghai, 200234, People's Republic of China.
CAS Key Laboratory of Genome Sciences and Information, Beijing Key Laboratory of Genome and Precision Medicine Technologies, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijng, 100101, People's Republic of China.
Biotechnol Lett. 2017 May;39(5):731-738. doi: 10.1007/s10529-017-2301-x. Epub 2017 Apr 21.
To improve H production, the green algae Chlamydomonas reinhardtii cc849 was co-cultured with Azotobacter chroococcum.
The maximum H production of the co-culture was 350% greater than that of the pure algal cultures under optimal H production conditions. The maximum growth and the respiratory rate of the co-cultures were about 320 and 300% of the controls, and the dissolved O of co-cultures was decreased 74%. Furthermore, the in vitro maximum hydrogenase activity of the co-culture was 250% greater than that of the control, and the in vivo maximum hydrogenase activity of the co-culture was 1.4-fold greater than that of the control. In addition, the maximum starch content of co-culture was 1400% that of the control.
Azotobacter chroococcum improved the H production of the co-cultures by decreasing the O content and increasing the growth and starch content of the algae and the hydrogenase activity of the co-cultures relative to those of pure algal cultures.
为提高氢气产量，将莱茵衣藻cc849与褐球固氮菌进行共培养。
在最佳产氢条件下，共培养物的最大产氢量比纯藻培养物高350%。共培养物的最大生长量和呼吸速率分别约为对照的320%和300%，共培养物中的溶解氧降低了74%。此外，共培养物的体外最大氢化酶活性比对照高250%，共培养物的体内最大氢化酶活性是对照的1.4倍。另外，共培养物的最大淀粉含量是对照的1400%。
相对于纯藻培养物，褐球固氮菌通过降低氧含量、增加藻类的生长和淀粉含量以及共培养物的氢化酶活性，提高了共培养物的产氢量。
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