Pioro E P, Cuello A C
Department of Pharmacology and Therapeutics, McGill University, Montreal, Que., Canada.
Brain Res. 1988 Jul 5;455(1):182-6. doi: 10.1016/0006-8993(88)90131-x.
Adult rat cerebellum was examined for the presence of nerve growth factor receptor (NGFr) immunoreactivity (IR) using the monoclonal antibody 192-IgG. In normal animals, light NGFr-IR was observed consistently in Purkinje cells and molecular layer throughout the flocculonodular lobe. However, animals treated with intracerebroventricular colchicine 7-72 h prior to analysis displayed a graded increase of IR in Purkinje cells of all regions with progressively longer survival times. These findings demonstrate the usefulness of colchicine in revealing accumulation of NGFr in adult CNS neurons where previously not visualized and suggest responsiveness of a non-cholinergic neuronal population to nerve growth factor (NGF).
使用单克隆抗体192-IgG检测成年大鼠小脑神经生长因子受体（NGFr）免疫反应性（IR）。在正常动物中，始终能在整个绒球小结叶的浦肯野细胞和分子层中观察到轻度的NGFr-IR。然而，在分析前7 - 72小时经脑室内注射秋水仙碱处理的动物，随着存活时间逐渐延长，所有区域的浦肯野细胞中IR呈分级增加。这些发现证明了秋水仙碱在揭示成年中枢神经系统神经元中NGFr积累方面的作用，此前这些积累未被观察到，并且提示了非胆碱能神经元群体对神经生长因子（NGF）的反应性。
