胆固醇与磷脂酰胆碱二元混合物中的横向扩散
Lateral diffusion in binary mixtures of cholesterol and phosphatidylcholines.
作者信息
Rubenstein J L, Smith B A, McConnell H M
出版信息
Proc Natl Acad Sci U S A. 1979 Jan;76(1):15-8. doi: 10.1073/pnas.76.1.15.
Abstract
The lateral diffusion of a fluorescent-labeled phospholipid, phosphatidyl-N-(4-nitrobenzo-2-oxa-1,3,-diazole)ethanolamine, has been measured in binary mixtures of cholesterol and dimyristoyl phosphatidylcholine at temperatures both above and below 23.8 degrees C, the chain-melting transition temperature of this phosphatidylcholine. There is a temperature-composition region, approximately temperature less than 23 degrees C and mole fraction of cholesterol (X) less than 0.20, in which the lateral diffusion coefficient of the fluorescent probe is at least an order of magnitude smaller than it is at points outside of this temperature-composition region. At temperatures above approximately 23 degrees C there is a significant increasing cholesterol concentration, for X greater than 0.2.
摘要
在高于和低于23.8℃（这种磷脂酰胆碱的链熔化转变温度）的温度下，已测量了荧光标记的磷脂——磷脂酰 - N -（4 - 硝基苯并 - 2 - 恶唑 - 1,3 - 二唑）乙醇胺在胆固醇和二肉豆蔻酰磷脂酰胆碱二元混合物中的横向扩散。存在一个温度 - 组成区域，大约温度低于23℃且胆固醇摩尔分数（X）小于0.20，在该区域中荧光探针的横向扩散系数比该温度 - 组成区域之外的点至少小一个数量级。在大约23℃以上的温度下，当X大于0.2时，胆固醇浓度显著增加。
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