Charlins Paige, Schmitt Kimberly, Remling-Mulder Leila, Hogan Louise E, Hanhauser Emily, Hobbs Kristen S, Hecht Frederick, Deeks Steven G, Henrich Timothy J, Akkina Ramesh
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA.
Division of Experimental Medicine, UCSF School of Medicine, San Francisco, CA 94110, USA; Division of Infectious Diseases, Brigham and Women's Hospital/Harvard Medical School, Boston, MA 02110, USA.
Virology. 2017 Jul;507:135-139. doi: 10.1016/j.virol.2017.04.011. Epub 2017 Apr 19.
Assays that can verify full viral eradication are essential in the context of achieving a cure for HIV/AIDS. In vitro quantitative viral out growth assays (qVOA) are currently the gold standard for measuring latent HIV-1 but these assays often fail to detect very low levels of replication-competent virus. Here we investigated an alternative in vivo approach for sensitive viral detection using humanized mice (hmVOA). Peripheral blood CD4+ T cell samples from HIV subjects on stable ART with undetectable viral loads by RT-PCR were first assayed by in vitro qVOA. Corresponding patient samples in which no virus was detected by qVOA were injected into humanized mice to allow viral outgrowth. Of the five qVOA virus negative samples, four gave positive viral outgrowth in the hmVOA assay suggesting that it is more sensitive in detecting latent HIV-1.
在实现治愈艾滋病毒/艾滋病的背景下，能够验证病毒完全根除的检测方法至关重要。体外定量病毒生长检测法（qVOA）目前是检测潜伏性HIV-1的金标准，但这些检测方法常常无法检测到极低水平的具有复制能力的病毒。在此，我们研究了一种使用人源化小鼠进行敏感病毒检测的体内替代方法（hmVOA）。首先通过体外qVOA对接受稳定抗逆转录病毒治疗（ART）且通过逆转录聚合酶链反应（RT-PCR）检测不到病毒载量的艾滋病毒感染者的外周血CD4 + T细胞样本进行检测。将qVOA检测未检测到病毒的相应患者样本注射到人源化小鼠体内，以促进病毒生长。在五个qVOA病毒阴性样本中，有四个在hmVOA检测中出现了阳性病毒生长，这表明hmVOA在检测潜伏性HIV-1方面更敏感。
