Department of Molecular and Comparative Pathobiology, Center for AIDS Research, The Johns Hopkins School of Medicine, Baltimore, MD, USA.
Department of Medicine, Center for AIDS Research, The Johns Hopkins School of Medicine, Baltimore, MD, USA.
Retrovirology. 2017 Nov 21;14(1):52. doi: 10.1186/s12977-017-0376-z.
Sensitive assays are needed for the detection of residual viral reservoirs in HIV-1-infected subjects on suppressive combination antiretroviral therapy regimens to determine whether eradication strategies are effective. Mouse viral outgrowth assays have recently been developed and have the potential to be more sensitive than traditional in vitro quantitative viral outgrowth assays. In this article we describe these assays and review several studies that have used them to measure the latent reservoir.
需要灵敏的检测方法来检测接受抑制性联合抗逆转录病毒治疗方案的 HIV-1 感染者体内的残留病毒库，以确定清除策略是否有效。最近已经开发出了小鼠病毒扩增检测方法，其灵敏度可能比传统的体外定量病毒扩增检测方法更高。本文将描述这些检测方法，并综述了一些使用这些方法来测量潜伏性病毒库的研究。
