Amati B B, Gasser S M
Swiss Institute for Experimental Cancer Research, Epalinges s/Lausanne.
Cell. 1988 Sep 23;54(7):967-78. doi: 10.1016/0092-8674(88)90111-0.
We describe here for the first time the isolation of a yeast nuclear scaffold that maintains specific interactions with yeast genomic DNA sequences. The scaffold-DNA interaction is reversible and saturable, and some binding sites are conserved between yeast and Drosophila KC cells. Second, we find that the specific sequences bound to the yeast nuclear scaffold are the putative origins of replication (ARS elements) and a chromosomal centromere, CENIII. The scaffold association has been closely mapped at the ARS1 locus, and appears to include the 11 bp ARS consensus, but not the ABF-1 binding site. Competition studies show that ARS1 does not compete for CENIII binding, allowing us to distinguish two classes of scaffold attachment sites by functional and structural criteria.
我们在此首次描述了一种酵母核支架的分离，该支架与酵母基因组DNA序列保持特定相互作用。支架与DNA的相互作用是可逆且可饱和的，并且酵母和果蝇KC细胞之间的一些结合位点是保守的。其次，我们发现与酵母核支架结合的特定序列是假定的复制起点（ARS元件）和一个染色体着丝粒CENIII。在ARS1位点已对支架关联进行了精细定位，并且似乎包括11 bp的ARS共有序列，但不包括ABF-1结合位点。竞争研究表明，ARS1不竞争CENIII结合，这使我们能够通过功能和结构标准区分两类支架附着位点。
