Dresler Sławomir, Wójciak-Kosior Magdalena, Sowa Ireneusz, Stanisławski Grzegorz, Bany Izabela, Wójcik Małgorzata
Department of Plant Physiology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland.
Department of Analytical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093, Lublin, Poland.
Plant Physiol Biochem. 2017 Jun;115:380-389. doi: 10.1016/j.plaphy.2017.04.016. Epub 2017 Apr 13.
The aim of the study was to determine the response of metallicolous and nonmetallicolous Echium vulgare L. populations to chronic multi-metal (Zn, Pb, Cd) and acute Zn (200, 400 μM) and Pb (30, 60 μM) stress. Three populations of E. vulgare, one from uncontaminated and two from metal-contaminated areas, were studied. Two types of experiments were performed - a short-term hydroponic experiment with acute Zn or Pb stress and a long-term manipulative soil experiment with the use of soils from the sites of origin of the three populations. Growth parameters, such as shoot and root fresh weight and leaf area, as well as organic acid accumulation were determined. Moreover, the concentration of selected secondary metabolites and antioxidant capacity in the three populations exposed to Pb or Zn excess were measured. Both metallicolous populations generally achieved higher biomass compared with the nonmetallicolous population cultivated under metal stress in hydroponics or on metalliferous substrates. Plants exposed to Pb or Zn excess or contaminated soil substrate exhibited higher malate and citrate concentrations compared with the reference (no metal stress) plants. It was observed that Zn or Pb stress increased accumulation of allantoin, chlorogenic and rosmarinic acids, total phenolics, and flavonoids. Moreover, it was shown that Pb sequestration in the roots or Zn translocation to the shoots may play a role in enhanced metal tolerance of metallicolous populations under acute Pb/Zn stress.
本研究的目的是确定嗜金属和非嗜金属的普通蓝蓟（Echium vulgare L.）种群对慢性多金属（锌、铅、镉）以及急性锌（200、400 μM）和铅（30、60 μM）胁迫的响应。对普通蓝蓟的三个种群进行了研究，其中一个来自未受污染地区，另外两个来自金属污染地区。进行了两种类型的实验——一个是急性锌或铅胁迫的短期水培实验，另一个是使用来自这三个种群原生地土壤的长期人工控制土壤实验。测定了生长参数，如地上部和根部鲜重、叶面积以及有机酸积累。此外，还测量了暴露于过量铅或锌的三个种群中选定次生代谢产物的浓度和抗氧化能力。与在水培中金属胁迫下或在含金属基质上培养的非嗜金属种群相比，两个嗜金属种群通常获得了更高的生物量。与对照（无金属胁迫）植物相比，暴露于过量铅或锌或受污染土壤基质的植物表现出更高的苹果酸和柠檬酸浓度。观察到锌或铅胁迫增加了尿囊素、绿原酸和迷迭香酸、总酚类和黄酮类的积累。此外，研究表明，在急性铅/锌胁迫下，根部对铅的螯合或锌向地上部的转运可能在嗜金属种群增强的金属耐受性中发挥作用。
