Dresler Sławomir, Hawrylak-Nowak Barbara, Strzemski Maciej, Wójciak-Kosior Magdalena, Sowa Ireneusz, Hanaka Agnieszka, Gołoś Iwona, Skalska-Kamińska Agnieszka, Cieślak Małgorzata, Kováčik Jozef
Department of Plant Physiology and Biophysics, Institute of Biological Science, Maria Curie-Skłodowska University, 20-033 Lublin, Poland.
Department of Botany and Plant Physiology, Faculty of Environmental Biology, University of Life Sciences in Lublin, Akademicka 15, 20-950 Lublin, Poland.
Plants (Basel). 2019 Nov 17;8(11):517. doi: 10.3390/plants8110517.
Silver is one of the most toxic heavy metals for plants, inducing various toxic symptoms and metabolic changes. Here, the impact of Ag(I) on physiology and selected metabolites was studied using two Ag concentrations (1 or 10 µM) after 14 days of exposure. The higher concentration of Ag(I) evoked reduction of growth, while 1 µM Ag had a growth-promoting effect on root biomass. The translocation factor (<0.04) showed that Ag was mainly retained in the roots. The 1 µM Ag concentration increased the level of low-molecular-weight organic acids (LMWOAs), while 10 µM Ag depleted these compounds in the roots. The increased concentration of Ag(I) elevated the accumulation of phytochelatins (PCs) in the roots and reduced glutathione (GSH) in the shoots (but not in the roots). At 1 µM, Ag(I) elevated the level of phenolic and triterpene acids, while the 10 µM Ag treatment increased the carlina oxide content in the roots. The obtained results indicate an alteration of metabolic pathways of to cope with different levels of Ag(I) stress. Our data imply that the intracellular binding of Ag(I) and nonenzymatic antioxidants contribute to the protection against low concentrations of Ag ions.
银是对植物毒性最强的重金属之一，会引发各种毒性症状和代谢变化。在此，在暴露14天后，使用两种银浓度（1或10 μM）研究了Ag(I)对生理和选定代谢物的影响。较高浓度的Ag(I)导致生长减少，而1 μM银对根生物量有促进生长的作用。转运因子（<0.04）表明银主要保留在根部。1 μM银浓度增加了低分子量有机酸（LMWOAs）的水平，而10 μM银使根部的这些化合物减少。Ag(I)浓度的增加提高了根部植物螯合肽（PCs）的积累，并降低了地上部分（但不是根部）的谷胱甘肽（GSH）。在1 μM时，Ag(I)提高了酚酸和三萜酸的水平，而10 μM银处理增加了根部氧化卡尔烯的含量。所得结果表明为应对不同水平的Ag(I)胁迫，代谢途径发生了改变。我们的数据表明，Ag(I)的细胞内结合和非酶抗氧化剂有助于抵御低浓度的银离子。
