The Petroleum and Petrochemical College, Chulalongkorn University, Bangkok 10330, Thailand.
The Petroleum and Petrochemical College, Chulalongkorn University, Bangkok 10330, Thailand; Center of Excellence on Petrochemical and Materials Technology, Chulalongkorn University, Bangkok 10330, Thailand; NU-PPC Plasma Chemical Technology Laboratory, Chulalongkorn University, Bangkok 10330, Thailand.
Carbohydr Polym. 2017 Jul 1;167:1-11. doi: 10.1016/j.carbpol.2017.03.006. Epub 2017 Mar 6.
Solution plasma (SP) treatment in combination with oxidizing agents, i.e., hydrogen peroxide (HO), potassium persulfate (KSO) and sodium nitrite (NaNO) were adopted to chitosan degradation in order to achieve fast degradation rate, low chemicals used and high yield of low-molecular-weight chitosan and chitooligosaccharide (COS). Among the studied oxidizing agents, HO was found to be the best choice in terms of appreciable molecular weight reduction without major change in chemical structure of the degraded products of chitosan. By the combination with SP treatment, dilute solution of HO (4-60mM) was required for effective degradation of chitosan. The combination of SP treatment and dilute solution of HO (60mM) resulted in the great reduction of molecular weight of chitosan and water-soluble chitosan was obtained as a major product. The resulting water-soluble chitosan was precipitated to obtain COS. An inhibitory effect against cervical cancer cell line (HeLa cells) of COS was also examined.
溶液等离子体 (SP) 处理与氧化剂（如过氧化氢 (HO)、过硫酸钾 (KSO) 和亚硝酸钠 (NaNO)）联合使用，以降解壳聚糖，从而实现快速降解率、使用低化学物质和获得高产率的低分子量壳聚糖和壳寡糖 (COS)。在研究的氧化剂中，HO 在不改变壳聚糖降解产物的化学结构的情况下，表现出显著的分子量降低，是最佳选择。通过与 SP 处理相结合，仅需低浓度的 HO（4-60mM）即可有效降解壳聚糖。SP 处理与低浓度 HO（60mM）的组合导致壳聚糖的分子量大大降低，主要产物为水溶性壳聚糖。所得水溶性壳聚糖沉淀后得到 COS。还研究了 COS 对宫颈癌细胞系 (HeLa 细胞) 的抑制作用。
