Abdel Rahman Rana M, Ghaffar Hedayat Abdel, Alkhuriji Afrah F, Khalil Mahmoud I, Amaly Noha, El-Kott Attalla F, Sultan Ahmed S
Zoology Department, Faculty of Science, Damanhour University, Damanhour, Egypt.
Zoology Department, Faculty of Science, Alexandria University, Alexandria, Egypt.
Evid Based Complement Alternat Med. 2020 Sep 28;2020:4396305. doi: 10.1155/2020/4396305. eCollection 2020.
Traditional folk therapies indicate that insects have diverse medicinal potentials. However, the therapeutic application of insect chitosan and its derivatives has not been explored. To investigate the application of chitosan and its derivatives, the carboxymethyl derivative of chitosan (CM-Ch) was extracted from two dipteran larvae species, and . The degree of deacetylation (DD) and CM-Ch functional groups were validated using Fourier-transform infrared (FTIR) spectroscopy analysis and proton nuclear magnetic resonance spectroscopy (H NMR), respectively. The molecular weight was estimated using MALDI-TOF MS analysis. The effect of CM-Ch on the morphology and proliferation of human liver HepG2 cancer cells was assessed. IC of CM-Ch induced significant growth-inhibitory effects in HepG2 cells. CM-Ch treatment altered the morphology of HepG2 in a dose-dependent manner and induced apoptosis in a caspase-dependent manner. CM-Ch treatment showed no signs of toxicity, and no alterations in liver and kidney biochemical markers were observed in albino rats. A CM-Ch derivative from commercial crustacean chitosan was used to assess the efficacy of the insect-derived CM-Ch. The data presented here introduce insect CM-Ch as a promising, inexhaustible, safe derivative of chitosan with antitumor potential in liver cancer. This is the first report highlighting the anticancer activity of insect CM-Ch in hepatocellular carcinoma cells.
传统民间疗法表明昆虫具有多种药用潜力。然而，昆虫壳聚糖及其衍生物的治疗应用尚未得到探索。为了研究壳聚糖及其衍生物的应用，从两种双翅目幼虫物种中提取了壳聚糖的羧甲基衍生物（CM-Ch）。分别使用傅里叶变换红外（FTIR）光谱分析和质子核磁共振光谱（H NMR）验证脱乙酰度（DD）和CM-Ch官能团。使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）分析估计分子量。评估了CM-Ch对人肝癌HepG2细胞形态和增殖的影响。CM-Ch的半数抑制浓度（IC）在HepG2细胞中诱导了显著的生长抑制作用。CM-Ch处理以剂量依赖的方式改变了HepG2的形态，并以半胱天冬酶依赖的方式诱导细胞凋亡。CM-Ch处理未显示出毒性迹象，在白化大鼠中未观察到肝脏和肾脏生化标志物的改变。使用来自商业甲壳类壳聚糖的CM-Ch衍生物来评估昆虫来源的CM-Ch的功效。此处提供的数据表明昆虫CM-Ch是一种有前景、取之不尽、安全的壳聚糖衍生物，在肝癌中具有抗肿瘤潜力。这是第一份突出昆虫CM-Ch在肝癌细胞中抗癌活性的报告。
