来自杜氏枪乌贼（Ortmann，1891）的生物活性壳聚糖和硫酸化壳聚糖的表征。
Characterization of bioactive chitosan and sulfated chitosan from Doryteuthis singhalensis (Ortmann, 1891).
作者信息
Ramasamy Pasiyappazham, Subhapradha Namasivayam, Thinesh Thangadurai, Selvin Joseph, Selvan Kanagaraj Muthamizh, Shanmugam Vairamani, Shanmugam Annaian
机构信息
Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, India; Department of Microbiology, School of Life Sciences, Pondicherry University, Puducherry 605 014, India.
Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, India; Faculty of Allied Health Sciences, Chettinad Academy of Research and Education, Kelambakkam, Chennai 603 103, India.
出版信息
Int J Biol Macromol. 2017 Jun;99:682-691. doi: 10.1016/j.ijbiomac.2017.03.041. Epub 2017 Mar 8.
Chitosan was extracted from the pen of squid Doryteuthis singhalensis and characterized using FT-IR, NMR, CHN, SEM and DSC analysis. Purified chitosan was sulfated with chlorosulfonic acid in N,N-dimethylformamide and the added sulfate group was confirmed with FT-IR analysis. The molecular weight and degree of deacetylation (DDA) of chitosan was found 226.6kDa and 83.76% respectively. Chitosan exhibited potent antioxidant activity evidenced by reducing power, chelating ability on ferrous ions and scavenging activity on DPPH, superoxide and hydroxyl radicals. The anticoagulant assay using activated partial thromboplastin time (APTT) and prothrombin time (PT) showed chitosan as a strong anticoagulant. The results of this study showed possibility of using D. singhalensis pen as a non-conventional source of natural antioxidants and anticoagulant which can be incorporated in functional food formulations.
从杜氏枪乌贼（Doryteuthis singhalensis）的笔中提取了壳聚糖，并使用傅里叶变换红外光谱（FT-IR）、核磁共振（NMR）、元素分析（CHN）、扫描电子显微镜（SEM）和差示扫描量热法（DSC）分析对其进行了表征。在N，N-二甲基甲酰胺中用氯磺酸将纯化的壳聚糖硫酸化，并用傅里叶变换红外光谱分析确认添加的硫酸根。壳聚糖的分子量和脱乙酰度（DDA）分别为226.6 kDa和83.76%。壳聚糖表现出强大的抗氧化活性，通过还原能力、对亚铁离子的螯合能力以及对二苯基苦味酰基自由基（DPPH）、超氧阴离子自由基和羟基自由基的清除活性得以证明。使用活化部分凝血活酶时间（APTT）和凝血酶原时间（PT）进行的抗凝试验表明壳聚糖是一种强效抗凝剂。本研究结果表明，杜氏枪乌贼的笔有可能作为天然抗氧化剂和抗凝剂的非常规来源，可纳入功能性食品配方中。
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