Li Qian, Chen Jun, Li Ti, Liu Chengmei, Wang Xieyi, Dai Taotao, McClements David Julian, Liu Jiyan
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, Jiangxi, PR China.
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, Jiangxi, PR China.
Food Res Int. 2015 Dec;78:378-387. doi: 10.1016/j.foodres.2015.09.004. Epub 2015 Sep 10.
The influence of passage through a simulated gastrointestinal tract (GIT) on the stability and bioaccessibility of proanthocyanidins isolated from fruit (Choerospondias axillaris) peel was studied. In addition, the effects of the simulated GIT on the antioxidant and α-glucosidase inhibitory activities of proanthocyanidins extracts were evaluated. Gastric digestion had little effect on total polyphenol content (TPC) or mean degree of polymerization (mDP) of crude and purified extracts. However, intestinal digestion led to a significant decrease (about 26% and 19%) in TPC and (about 12% and 7%) in mDP for crude and purified extracts, respectively. The observed reduction in TPC and mDP levels was attributed to interactions of proanthocyanidins with pancreatic enzymes, rather than due to the chemical conditions during digestion. Only small flavan-3-ol molecules (monomers, dimers and trimers) could diffuse into the dialysis tubing used to simulate the intestinal wall. Changes in antioxidant activity during digestion were correlated to changes in TPC. After simulated GIT digestion, over 85% of α-glucosidase inhibitory activity of both extracts was preserved. These results indicate that the majority of the proanthocyanidins maintained their biological activities after passage through the simulated GIT, and were therefore still capable of providing valuable health benefits.
研究了通过模拟胃肠道（GIT）对从水果（南酸枣）果皮中分离的原花青素的稳定性和生物可及性的影响。此外，还评估了模拟GIT对原花青素提取物的抗氧化和α-葡萄糖苷酶抑制活性的影响。胃消化对粗提物和纯化提取物的总多酚含量（TPC）或平均聚合度（mDP）影响较小。然而，肠道消化导致粗提物和纯化提取物的TPC分别显著降低（约26%和19%），mDP分别显著降低（约12%和7%）。观察到的TPC和mDP水平的降低归因于原花青素与胰酶的相互作用，而不是消化过程中的化学条件。只有小的黄烷-3-醇分子（单体、二聚体和三聚体）能够扩散到用于模拟肠壁的透析管中。消化过程中抗氧化活性的变化与TPC的变化相关。经过模拟GIT消化后，两种提取物超过85%的α-葡萄糖苷酶抑制活性得以保留。这些结果表明，大多数原花青素在通过模拟GIT后仍保持其生物活性，因此仍能够提供有价值的健康益处。
