College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen 518060, PR China; State Key Laboratory of Marine Environmental Science, Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem, College of the Environment and Ecology, Xiamen University, Xiamen 361005, PR China.
State Key Laboratory of Marine Environmental Science, Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem, College of the Environment and Ecology, Xiamen University, Xiamen 361005, PR China.
Bioresour Technol. 2017 Aug;238:164-173. doi: 10.1016/j.biortech.2017.04.018. Epub 2017 Apr 8.
Naproxen is one of the most prevalent pharmaceuticals and of great environment concern. Information about bioremediation of naproxen by algae remains limited and no study has been reported on the degradation mechanism and the toxicity of NPX on algae. In this study, both Cymbella sp. and Scenedesmus quadricauda showed complete growth inhibition (100%) at 100mgL within 24h. Biochemical characteristics including chlorophyll a, carotenoid contents and enzyme activities for these two microalgae were affected by NPX at relatively high concentrations after 4d of exposure. Degradation of naproxen was accelerated by both algae species. Cymbella sp. showed a more satisfactive effect in the bioremediation of NPX with higher removal efficiency. A total of 12 metabolites were identified by LC-MS/MS and the degradation pathways of naproxen in two algae were proposed. Hydroxylation, decarboxylation, demethylation, tyrosine conjunction and glucuronidation contributed to naproxen transformation in algal cells.
萘普生是最常见的药物之一，也是人们非常关注的环境污染物。有关藻类对萘普生的生物修复信息仍然有限，并且尚未有研究报道 NPX 对藻类的降解机制和毒性。在这项研究中，在 24 小时内，Cymbella sp. 和 Scenedesmus quadricauda 在 100mg/L 时完全抑制生长（100%）。在暴露 4 天后，相对较高浓度的 NPX 会影响这两种微藻的生化特性，包括叶绿素 a、类胡萝卜素含量和酶活性。萘普生的降解被两种藻类加速。Cymbella sp. 在 NPX 的生物修复中表现出更令人满意的效果，具有更高的去除效率。通过 LC-MS/MS 共鉴定出 12 种代谢物，并提出了萘普生在两种藻类中的降解途径。羟基化、脱羧、去甲基、酪氨酸结合和葡萄糖醛酸化有助于萘普生在藻类细胞中的转化。
