Murphy T F, Bartos L C
Department of Medicine, State University of New York, Buffalo 14215.
Infect Immun. 1988 Oct;56(10):2673-9. doi: 10.1128/iai.56.10.2673-2679.1988.
The human bactericidal antibody response to the major outer membrane protein, P2, of nontypeable Haemophilus influenzae was studied. P2 was isolated from two strains of nontypeable H. influenzae and coupled to affinity columns. Pooled normal human serum was subjected to affinity chromatography with the P2 columns and a control column. Reducing the titer of antibody to P2 resulted in reduced bactericidal activity of that serum for the organism. Immunopurified antibody to P2 from human serum was bactericidal for the homologous strain. The extent to which these bactericidal determinants on P2 are conserved among strains was investigated. Immunopurified antibodies to P2 of two epidemiologically unrelated isolates were bactericidal for four of six strains tested. We conclude that P2 is a target for human bactericidal antibody and that some of these determinants that are recognized by human bactericidal antibody are conserved among strains of nontypeable H. influenzae.
研究了人类针对不可分型流感嗜血杆菌主要外膜蛋白P2的杀菌抗体反应。从两株不可分型流感嗜血杆菌中分离出P2，并将其偶联到亲和柱上。将混合的正常人血清用P2柱和对照柱进行亲和层析。降低血清中针对P2的抗体滴度会导致该血清对该微生物的杀菌活性降低。从人血清中免疫纯化的针对P2的抗体对同源菌株具有杀菌作用。研究了P2上这些杀菌决定簇在菌株间的保守程度。针对两种流行病学上不相关分离株的P2的免疫纯化抗体对所测试的六株菌株中的四株具有杀菌作用。我们得出结论，P2是人类杀菌抗体的靶标，并且人类杀菌抗体识别的一些决定簇在不可分型流感嗜血杆菌菌株间是保守的。
