Barenkamp S J, Munson R S, Granoff D M
Infect Immun. 1982 May;36(2):535-40. doi: 10.1128/iai.36.2.535-540.1982.
The techniques of biotype determination and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of outer membrane protein preparations were applied to 35 epidemiologically unrelated isolates of pathogenic nontypable Haemophilus influenzae. Three of five isolates obtained from the blood of unrelated newborns with sepsis had concordant major outer membrane from the blood of unrelated older children or adults with bacteremia had concordant major outer membrane protein profiles, distinct from the common profile of neonatal strains, and were biotype II. The outer membrane protein profiles of the remaining 5 isolates from blood, 2 isolated from cerebrospinal fluid, and 23 isolated from middle ear aspirates of children with otitis media were unique, although each isolate had peptides with apparent molecular weights of 16,000 and 31,500. These results suggest that a subset of nontypable isolates associated with bacteremia has distinctive strain markers. Their pathogenicity may relate to a prediction for colonizing the female genital tract in the case of the common neonatal strain or an increased ability to evade host defenses.
生物型测定技术以及外膜蛋白制剂的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳技术被应用于35株流行病学上无关联的致病性不可分型流感嗜血杆菌分离株。从患败血症的无关联新生儿血液中分离出的5株菌中有3株具有一致的主要外膜蛋白谱型；从患菌血症的无关联大龄儿童或成人血液中分离出的菌株具有一致的主要外膜蛋白谱型，与新生儿菌株的常见谱型不同，且属于生物型II。其余5株血液分离株、2株脑脊液分离株以及23株从中耳炎患儿中耳吸出物中分离出的菌株，其外膜蛋白谱型是独特的，尽管每株分离株都有表观分子量为16,000和31,500的肽段。这些结果表明，与菌血症相关的一部分不可分型分离株具有独特的菌株标记。它们的致病性可能与常见新生儿菌株在女性生殖道定植的倾向有关，或者与逃避宿主防御的能力增强有关。
