Pagnani M, Corallini A, Caputo A, Altavilla G, Selvatici R, Catozzi L, Possati L, Barbanti-Brodano G
Institute of Microbiology, School of Medicine, University of Ferrara, Italy.
Int J Cancer. 1988 Sep 15;42(3):405-13. doi: 10.1002/ijc.2910420317.
Early-passage hamster embryo cells were transformed by recombinant DNA molecules containing BK virus (BKV) early-region gene and either the activated c-Ha-ras oncogene (pBK/c-rasA) or the normal c-Ha-ras proto-oncogene (pBK/c-rasN). The recombinant DNAs had a greater transforming ability and converted hamster cells to a more malignant phenotype than the single genes transfected separately. pBK/c-rasA was significantly more powerful than pBK/c-rasN in conferring to cells all the characteristics of transformation. Transfected DNA sequences were integrated mostly as single insertions into cellular DNA. Specific c-Ha-ras and BKV transcripts as well as c-Ha-ras p21 and BKV T antigen were detected in transformed cells. Although stimulation of c-Ha-ras expression by BKV enhancers cannot be excluded in recombinants, super-transfection and co-transfection experiments in hamster embryo cells and pre-neoplastic cell lines showed that BKV early-region and c-Ha-ras co-operate in transformation by contributing separate and independent functions.
早期传代的仓鼠胚胎细胞被含有BK病毒（BKV）早期区域基因以及激活的c-Ha-ras癌基因（pBK/c-rasA）或正常c-Ha-ras原癌基因（pBK/c-rasN）的重组DNA分子转化。与单独转染的单个基因相比，重组DNA具有更强的转化能力，并将仓鼠细胞转变为更具恶性的表型。在赋予细胞所有转化特征方面，pBK/c-rasA比pBK/c-rasN显著更强。转染的DNA序列大多以单拷贝形式整合到细胞DNA中。在转化细胞中检测到了特异性的c-Ha-ras和BKV转录本以及c-Ha-ras p21和BKV T抗原。尽管在重组体中不能排除BKV增强子对c-Ha-ras表达的刺激作用，但在仓鼠胚胎细胞和肿瘤前细胞系中的超转染和共转染实验表明，BKV早期区域和c-Ha-ras通过发挥各自独立的功能在转化过程中协同作用。
