Corallini A, Pagnani M, Caputo A, Negrini M, Altavilla G, Catozzi L, Barbanti-Brodano G
Institute of Microbiology, School of Medicine, University of Ferrara, Italy.
J Gen Virol. 1988 Oct;69 ( Pt 10):2671-9. doi: 10.1099/0022-1317-69-10-2671.
Rapidly growing, undifferentiated brain tumours were induced in newborn Syrian hamsters by intracerebral inoculation of a recombinant DNA (pBK/c-rasA) carrying the BK virus (BKV) early region gene and the activated human c-Harvey-ras (c-Ha-ras) oncogene. Neither of the two genes inoculated alone nor recombinant DNA of the BKV early region gene and the normal human c-Ha-ras proto-oncogene were tumourigenic. Tumour-derived cell lines propagated in culture were immortalized and had growth characteristics consistent with a fully transformed phenotype. Tumours and tumour cell lines contained pBK/c-rasA sequences integrated into cellular DNA and expressed BKV- and c-Ha-ras-specific transcripts as well as BKV T antigen and c-Ha-ras p21. These findings are discussed in relation to a possible cooperation or synergism between BKV and cellular oncogenes in human neoplasia.
通过向新生叙利亚仓鼠脑内接种携带BK病毒（BKV）早期区域基因和激活的人c-Harvey-ras（c-Ha-ras）癌基因的重组DNA（pBK/c-rasA），诱导出快速生长的未分化脑肿瘤。单独接种的两个基因以及BKV早期区域基因与正常人c-Ha-ras原癌基因的重组DNA均无致瘤性。在培养中传代的肿瘤衍生细胞系永生化，具有与完全转化表型一致的生长特性。肿瘤和肿瘤细胞系含有整合到细胞DNA中的pBK/c-rasA序列，并表达BKV和c-Ha-ras特异性转录本以及BKV T抗原和c-Ha-ras p21。结合BKV与细胞癌基因在人类肿瘤形成中可能的协同作用或协同效应，对这些发现进行了讨论。
