College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu, PR China; College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, Henan, PR China.
College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, Henan, PR China.
Neuropeptides. 2017 Aug;64:47-60. doi: 10.1016/j.npep.2017.04.003. Epub 2017 Apr 6.
Neuromedin U (NMU) is a highly conserved neuropeptide that performs a variety of physiological functions in animals via neuromedin U receptor-1 (NMUR1) and neuromedin U receptor-2 (NMUR2). In this study, we cloned the pig NMU, NMUR1 and NMUR2 genes. Bioinformatics analysis demonstrated that the pig NMU cDNA encoded the amino acids Phe-Leu-Phe-Arg-Pro-Arg-Asn-NH2 at the C-terminus and that the NMU receptors, which are G-protein-coupled receptors (GPCRs), contained the seven transmembrane domains typical of GPCRs. Systemic NMU, NMUR1 and NMUR2 mRNA expression was investigated in various pig tissues using real-time RT-PCR. NMU mRNA was expressed both in the central nervous system (CNS) and in peripheral tissues. NMUR1 mRNA was widely expressed in peripheral tissues, whereas NMUR2 mRNA was mainly expressed in the CNS. Immunohistochemistry (IHC) was used to determine the expression patterns of NMU and NMUR1, which were predominantly located in the gastrointestinal tract, genitourinary organs, and immune organs. This study presents molecular and morphological data to aid in additional NMU research in pigs.
神经调节素 U（NMU）是一种高度保守的神经肽，通过神经调节素 U 受体-1（NMUR1）和神经调节素 U 受体-2（NMUR2）在动物中发挥多种生理功能。在本研究中，我们克隆了猪 NMU、NMUR1 和 NMUR2 基因。生物信息学分析表明，猪 NMU cDNA 编码 C 末端的氨基酸 Phe-Leu-Phe-Arg-Pro-Arg-Asn-NH2，而 NMU 受体是 G 蛋白偶联受体（GPCR），包含 GPCR 典型的七个跨膜结构域。使用实时 RT-PCR 研究了猪不同组织中系统 NMU、NMUR1 和 NMUR2 的 mRNA 表达。NMU mRNA 在中枢神经系统（CNS）和外周组织中均有表达。NMUR1 mRNA 在外周组织中广泛表达，而 NMUR2 mRNA 主要在 CNS 中表达。免疫组织化学（IHC）用于确定 NMU 和 NMUR1 的表达模式，它们主要位于胃肠道、泌尿生殖器官和免疫器官中。本研究提供了分子和形态学数据，以辅助猪 NMU 的进一步研究。
