中国南方鼬獾（Melogale moschata）弓形虫的PCR-RFLP检测及基因特征分析
Prevalence and genetic characterization of Toxoplasma gondii in badgers (Melogale moschata) in southern China by PCR-RFLP.
作者信息
Chen Kai, Huang Si-Yang, Wang Jin-Lei, Hu Rong-Liang, Yao Qiu-Xia, Zhang Shou-Feng, Zhu Xing-Quan, Liu Quan
机构信息
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China.
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China; Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University College of Veterinary Medicine, Yangzhou, Jiangsu Province 225009, PR China.
出版信息
Infect Genet Evol. 2017 Aug;52:30-33. doi: 10.1016/j.meegid.2017.04.019. Epub 2017 Apr 21.
Toxoplasma gondii is an obligate intracellular apicomplexan parasite which is able to infect almost all warm-blooded animals. There is no information about the prevalence and genetic characterization of T. gondii in badgers (Melogale moschata) in China. Here, a total of 367 badgers were captured from different cities in Jiangxi province, Southern China. Genomic DNA was extracted from brain tissues of each badgers, and 57 (15.45%) of them were positive for T. gondii by semi-nested PCR of the B1 gene. The positive DNA samples were typed at 11 genetic markers, including 10 nuclear loci (SAG1, 5'-SAG2 and 3'-SAG2, alternative SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1) and an apicoplast locus (Apico), with multilocus polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology. Among them, 4 were completely typed at all loci, and 2 was genotyped for 9 loci, showing that they belong to ToxoDB#9. This is the first report of prevalence and genetic characterization of T. gondii isolates from badgers in China, which contributes to broader understanding of population structure of T. gondii in China. It is important for the prevention and control of T. gondii infection in wild animals.
刚地弓形虫是一种专性细胞内顶复门寄生虫，能够感染几乎所有温血动物。在中国，关于猪獾（鼬獾）体内刚地弓形虫的流行情况和基因特征尚无相关信息。在此，共从中国南方江西省的不同城市捕获了367只猪獾。从每只猪獾的脑组织中提取基因组DNA，通过B1基因的半巢式PCR检测，其中57只（15.45%）猪獾的检测结果为刚地弓形虫阳性。利用多位点聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术，对阳性DNA样本在11个基因标记处进行分型，包括10个核基因座（SAG1、5'-SAG2和3'-SAG2、替代SAG2、SAG3、BTUB、GRA6、c22-8、c29-2、L358、PK1）和一个质体基因座（Apico）。其中，4个样本在所有基因座都完成了分型，2个样本在9个基因座进行了基因分型，结果显示它们属于ToxoDB#9型。这是中国首次关于猪獾体内刚地弓形虫分离株的流行情况和基因特征的报道，有助于更广泛地了解中国刚地弓形虫的种群结构。这对于野生动物中刚地弓形虫感染的预防和控制具有重要意义。
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