Zhou Dong-Hui, Zheng Wen-Bin, Hou Jun-Ling, Ma Jian-Gang, Zhang Xiao-Xuan, Zhu Xing-Quan, Cong Wei
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China.
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China; College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin Province 130118, PR China.
Acta Trop. 2017 Aug;172:143-146. doi: 10.1016/j.actatropica.2017.05.001. Epub 2017 May 2.
Toxoplasma gondii is a successful opportunistic parasite, affecting a wide range of vertebrate animals and humans. Genetic diversity of T. gondii in raccoon dogs (Nyctereutes procyonoides) is of great importance to understand the transmission of T. gondii in the environment. However, no information is available about the distribution of genetic diversity of T. gondii infection in raccoon dogs. This study was conducted to estimate the prevalence and genetic characterization of T. gondii from raccoon dogs in Shandong province, eastern China. A total of 314 brain tissue samples of raccoon dogs were collected and genomic DNA was extracted and assayed for T. gondii infection using semi-nested PCR targeting B1 gene. The positive DNA samples were typed at 10 genetic markers (SAG1, SAG2(5'+3' SAG2, alter.SAG2), SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico) by multiplex multilocus nested polymerase chain reaction-restriction fragment length polymorphism (Mn- PCR-RFLP) technology. Thirty-five (11.15%) of 314 DNA samples were detected positive. Only six samples were completely typed at all genetic loci, and these samples represented ToxoDB genotype#9. Two sample were typed at 9 genetic loci and one was typed at 8 genetic loci, all of them represented Type I. To our knowledge, this is the first report of genetic characterization of T. gondii in raccoon dogs in China. These results revealed the existence of genetic diversity of T. gondii in raccoon dogs in China. These data provided base-line information for controlling T. gondii infection in raccoon dogs.
刚地弓形虫是一种成功的机会性寄生虫，可感染多种脊椎动物和人类。了解貉（Nyctereutes procyonoides）体内刚地弓形虫的遗传多样性对于理解刚地弓形虫在环境中的传播至关重要。然而，目前尚无关于貉感染刚地弓形虫的遗传多样性分布的信息。本研究旨在估计中国东部山东省貉体内刚地弓形虫的感染率及其遗传特征。共收集了314份貉脑组织样本，提取基因组DNA，并使用靶向B1基因的半巢式PCR检测刚地弓形虫感染情况。对阳性DNA样本采用多重多位点巢式聚合酶链反应-限制性片段长度多态性（Mn-PCR-RFLP）技术在10个遗传标记（SAG1、SAG2（5'+3'SAG2、alter.SAG2）、SAG3、BTUB、GRA6、c22-8、c29-2、L358、PK1和Apico）上进行分型。314份DNA样本中有35份（11.15%）检测为阳性。只有6份样本在所有遗传位点上都完全分型，这些样本代表ToxoDB基因型#9。2份样本在9个遗传位点上分型，1份样本在8个遗传位点上分型，所有这些样本均代表I型。据我们所知，这是中国关于貉体内刚地弓形虫遗传特征的首次报道。这些结果揭示了中国貉体内刚地弓形虫存在遗传多样性。这些数据为控制貉感染刚地弓形虫提供了基线信息。
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