Hamid S, Bhat Mohd Altaf, Mir Irfan Ahmad, Taku Anil, Badroo Gulzar Ahmad, Nazki Salik, Malik Andleeb
Bacteriology Laboratory, Division of Veterinary Microbiology and Immunology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Jammu and Kashmir, India.
Laboratory of Veterinary Immunology, College of Veterinary Medicine, Chonbuk National University, Iksan, South Korea.
Vet World. 2017 Mar;10(3):363-367. doi: 10.14202/vetworld.2017.363-367. Epub 2017 Mar 30.
This study was conducted to determine the occurrence of methicillin-sensitive and (MSSA) methicillin-resistant (MRSA) from bovine mastitis and to characterize them with respect to antibiotic resistance gene .
A total of 160 mastitic milk samples were screened for the presence of . The presumptive positive isolates were confirmed using and gene-based polymerase chain reaction. All the confirmed isolates were subjected to antibiogram using a number of antibiotics. Isolates which showed resistance against methicillin were characterized for the presence of gene.
Out of the total 160 milk samples, 36 (22.5%) samples yielded . The antibiogram revealed that 16.6% isolates were resistant to all antibiotics screened for and 5.5% isolates were sensitive to all of them. Furthermore, the study found 94.4%, 83.3%, 77.7%, 66.6%, 50%, and 27.7% of isolates resistant to penicillin, ampicillin, amoxicillin-sulbactam, enrofloxacin, ceftriaxone, and methicillin, respectively. Out of the 36 isolates, only 6 (16.6%) isolates were confirmed as MRSA while rest were MSSA.
The higher occurrence of -mediated mastitis was concluded due to improper hygienic and poor farm management. The multiple drug resistance reveals the indiscriminate use of drugs and presence of methicillin resistance gene determinant is an alarming situation as such infections are difficult to treat.
本研究旨在确定奶牛乳腺炎中耐甲氧西林金黄色葡萄球菌（MSSA）和耐甲氧西林金黄色葡萄球菌（MRSA）的发生率，并对其抗生素耐药基因进行表征。
共筛选160份乳腺炎乳样以检测是否存在[相关细菌]。使用基于[特定基因]和[另一特定基因]的聚合酶链反应对推定阳性分离株进行确认。所有确认的分离株使用多种抗生素进行药敏试验。对显示对甲氧西林耐药的分离株进行[特定基因]检测。
在总共160份乳样中，36份（22.5%）样本检出[相关细菌]。药敏试验结果显示，16.6%的[相关细菌]分离株对所有筛选的抗生素耐药，5.5%的分离株对所有抗生素敏感。此外，研究发现分别有94.4%、83.3%、77.7%、66.6%、50%和27.7%的[相关细菌]分离株对青霉素、氨苄西林、阿莫西林 - 舒巴坦、恩诺沙星、头孢曲松和甲氧西林耐药。在36株[相关细菌]分离株中，只有6株（16.6%）被确认为MRSA，其余为MSSA。
由于卫生条件差和农场管理不善，得出[相关细菌]介导的乳腺炎发生率较高的结论。多重耐药性表明药物的滥用，而甲氧西林耐药基因决定因素的存在是一个令人担忧的情况，因为此类感染难以治疗。
