Multi Locus Sequence Typing and Typing of Isolated from the Milk of Cows with Subclinical Mastitis in Croatia.

BACKGROUND

The bacterial species is the most common causative agent of mastitis in cows in most countries with a dairy industry. The prevalence of infection caused by ranges from 2% to more than 50%, and it causes 10-12% of all cases of clinical mastitis.

AIM

The objective was to analyze 237 strains of isolated from the milk of cows with subclinical mastitis regarding the , and genes and to perform and multi-locus sequence typing (MLST).

METHODS

Sequencing amplified gene sequences was conducted at Macrogen Europe. Ridom StaphType and BioNumerics software was used to analyze obtained sequences of and seven housekeeping genes.

RESULTS

The fragment was present in 204 (86.1%) of strains, while and gene were detected in 10 strains, and the gene was not detected. typing successfully analyzed 153 tested isolates (64.3%), confirming 53 types, four of which were new types. The most frequent type was t2678 (14%). MLST typed 198 (83.5%) tested strains and defined 32 different allele profiles, of which three were new. The most frequent allele profile was ST133 (20.7%). Six groups (G) and 15 singletons were defined.

CONCLUSION

Taking the number of confirmed types and sequence types (STs) into account, it can be concluded that the strains of isolated from the milk of cows with subclinical mastitis form a heterogenous group. To check the possible zoonotic potential of isolates it would be necessary to test the persons and other livestock on the farms.