Rasyid H N, Soegijoko S
Department of Orthopaedics and Traumatology, Faculty of Medicine Universitas Padjadjaran / Dr. Hasan Sadikin General Hospital, Bandung, Indonesia.
Biomedical Engineering Program, School of Electrical Engineering and Informatics, Institut Teknologi Bandung, Indonesia.
Malays Orthop J. 2016 Jul;10(2):6-10. doi: 10.5704/MOJ.1607.002.
There have been many investigations on non-biodegradable materials acting as an antibiotic carrier for local drug delivery systems based on polymethyl methacrylate (PMMA) beads. However, the material is non-degradable and non-porous so that less than 5% of the encapsulated drug is released. In order to obtain better release of the antibiotics, greater porosity of the beads would be required. Adding fillers could increase the bead's porosity, thus improving the antibiotic release from the beads. The purpose of the study is to optimize release kinetics of gentamicin from handmade beads by adding fillers such as glycine and sodium chloride in different concentrations. Terms of percolation theory will qualitatively be applied in interpreting the final results. Model beads were made by blending the antibiotics (gentamicin) with powdered PMMA, prepared with the inclusion of glycine and different concentration of sodium chloride in 100% monomer. To determine the gentamicin release, beads were placed in phosphate buffered saline (PBS) and aliquots were taken at designated times to measure the gentamicin concentration. Addition of glycine yielded 16 % release of the total amount of gentamicin incorporated in 24 hours. Subsequent addition of sodium chloride resulted in an increased gentamicin release, with little or no difference in gentamicin release once 16 g or more sodium chloride was added (gentamicin release 100% of the amount incorporated). In conclusion, addition of glycine and sodium chloride resulted in an increased release of gentamicin; however, the combination without sodium chloride seemed to have an inhibitory effect on the gentamicin release.
针对基于聚甲基丙烯酸甲酯（PMMA）珠的局部给药系统中用作抗生素载体的不可生物降解材料，已经开展了许多研究。然而，该材料不可降解且无孔隙，以至于包封药物的释放量不到5%。为了使抗生素获得更好的释放效果，需要提高珠的孔隙率。添加填料可以增加珠的孔隙率，从而改善抗生素从珠中的释放。本研究的目的是通过添加不同浓度的甘氨酸和氯化钠等填料，优化手工制作的珠中庆大霉素的释放动力学。渗流理论术语将定性地用于解释最终结果。通过将抗生素（庆大霉素）与粉末状PMMA混合制作模型珠，在100%单体中加入甘氨酸和不同浓度的氯化钠来制备。为了测定庆大霉素的释放，将珠置于磷酸盐缓冲盐水（PBS）中，并在指定时间取等分试样以测量庆大霉素浓度。添加甘氨酸后，24小时内庆大霉素的总掺入量释放了16%。随后添加氯化钠导致庆大霉素释放增加，一旦添加16 g或更多氯化钠，庆大霉素释放几乎没有差异（庆大霉素释放量为掺入量的100%）。总之，添加甘氨酸和氯化钠导致庆大霉素释放增加；然而，不添加氯化钠的组合似乎对庆大霉素释放有抑制作用。
