Comparison of Differentiation of Induced Pluripotent Stem Cells and Bone-Marrow Mesenchymal Stem Cells to Osteoblast: Osteogenesis versus Pluripotency.

BACKGROUND

Derivation of induced pluripotent stem cells (iPSCs) from various adult somatic cells through over-expression of pluripotent genes could allow for the unlimited autologous supply in regenerative medicine. On the other hand the generation of various progenitors from bone-marrow mesenchymal stem cells (MSCs) is justly well established.

OBJECTIVE

In this study we compared the expression level of pluripotent genes and in iPSCs and MSCs derived from bone marrow. Also the potential of osteogenesis of iPSCs and bone-marrow MSCs were compared.

METHODS

We analyzed the expression level of and genes in human MSCs derived from iPSCs and MSCs by cell culture and real-time PCR. Also the expression level of and in both groups were evaluated.

RESULTS

We found that the expression of osteogenic markers in differentiated iPSCs to osteoblast were higher than bone-marrow MSCs. While the levels of pluripotency genes and in iPSCs were significantly (p<0.05) higher than bone-marrow MSCs, MSCs showed higher expression of and compared with iPSCs (p=NS).

CONCLUSION

It seems that the higher expression of and in MSCs compared to iPSCs may be due to other factors (besides pluripotency) required for differentiation of stem cells to osteoblast.