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诱导多能干细胞与骨髓间充质干细胞向成骨细胞分化的比较：成骨作用与多能性

Comparison of Differentiation of Induced Pluripotent Stem Cells and Bone-Marrow Mesenchymal Stem Cells to Osteoblast: Osteogenesis versus Pluripotency.

作者信息

Foroutan T

机构信息

Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.

出版信息

Int J Organ Transplant Med. 2016;7(2):91-96. Epub 2016 May 1.

PMID:28435641
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5396057/
Abstract

BACKGROUND

Derivation of induced pluripotent stem cells (iPSCs) from various adult somatic cells through over-expression of pluripotent genes could allow for the unlimited autologous supply in regenerative medicine. On the other hand the generation of various progenitors from bone-marrow mesenchymal stem cells (MSCs) is justly well established.

OBJECTIVE

In this study we compared the expression level of pluripotent genes and in iPSCs and MSCs derived from bone marrow. Also the potential of osteogenesis of iPSCs and bone-marrow MSCs were compared.

METHODS

We analyzed the expression level of and genes in human MSCs derived from iPSCs and MSCs by cell culture and real-time PCR. Also the expression level of and in both groups were evaluated.

RESULTS

We found that the expression of osteogenic markers in differentiated iPSCs to osteoblast were higher than bone-marrow MSCs. While the levels of pluripotency genes and in iPSCs were significantly (p<0.05) higher than bone-marrow MSCs, MSCs showed higher expression of and compared with iPSCs (p=NS).

CONCLUSION

It seems that the higher expression of and in MSCs compared to iPSCs may be due to other factors (besides pluripotency) required for differentiation of stem cells to osteoblast.

摘要

背景

通过多能基因的过表达从各种成体体细胞中诱导产生诱导多能干细胞（iPSC），这在再生医学中可实现无限的自体供应。另一方面，从骨髓间充质干细胞（MSC）生成各种祖细胞的方法已经得到充分确立。

目的

在本研究中，我们比较了源自骨髓的iPSC和MSC中多能基因的表达水平。此外，还比较了iPSC和骨髓MSC的成骨潜力。

方法

我们通过细胞培养和实时PCR分析了源自iPSC和MSC的人MSC中基因的表达水平。此外，还评估了两组中基因的表达水平。

结果

我们发现，分化为成骨细胞的iPSC中成骨标志物的表达高于骨髓MSC。虽然iPSC中多能基因的水平显著高于骨髓MSC（p<0.05），但与iPSC相比，MSC中基因的表达更高（p=无显著性差异）。

结论

与iPSC相比，MSC中基因的较高表达似乎可能是由于干细胞分化为成骨细胞所需的其他因素（除多能性外）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9edb/5396057/3f8d29d8479b/ijotm-7-091-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9edb/5396057/d1b08002280a/ijotm-7-091-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9edb/5396057/6de25fa50565/ijotm-7-091-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9edb/5396057/cf0bd69466b0/ijotm-7-091-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9edb/5396057/3f8d29d8479b/ijotm-7-091-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9edb/5396057/d1b08002280a/ijotm-7-091-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9edb/5396057/6de25fa50565/ijotm-7-091-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9edb/5396057/cf0bd69466b0/ijotm-7-091-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9edb/5396057/3f8d29d8479b/ijotm-7-091-g004.jpg

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