Vaidya Gayatri, Naik G R
Department of Biotechnology, Gulbarga University, Gulbarga 585106, India.
Biotechnol Rep (Amst). 2014 Aug 15;4:56-59. doi: 10.1016/j.btre.2014.08.003. eCollection 2014 Dec.
Due to lack of morphological methods to identify sex at early stage in the plants with long juvenile period the application of molecular markers is expected to facilitate breeding program. The objective of this study is to identify molecular markers linked to sex determination of the plant which assists in crop improvement program. Random amplified polymorphic DNA primers were tested on dioeceious and hermaphrodite plant . A set of eighty five RAPD primers were screened out of which only five primers were found to be associated with sex. The primer OPU-10 is male specific and OPD-19 primer is female specific. Another primer OPU-19 produced a unique amplification in only hermaphrodite individuals. Female and hermaphrodite specific primer OPS-05 amplified an amplicon in female and hermaphrodite and was absent in male plant. Primer OPW-03 produced amplicon specific to male and hermaphrodite plants and was absent in female plants.
由于缺乏在幼年期较长的植物中早期鉴定性别的形态学方法，分子标记的应用有望促进育种计划。本研究的目的是鉴定与该植物性别决定相关的分子标记，以协助作物改良计划。在雌雄异株和雌雄同株植物上测试了随机扩增多态性DNA引物。筛选出了85个RAPD引物，其中只有5个引物与性别相关。引物OPU - 10是雄性特异的，引物OPD - 19是雌性特异的。另一个引物OPU - 19仅在雌雄同株个体中产生独特的扩增。雌性和雌雄同株特异引物OPS - 05在雌性和雌雄同株中扩增出一个扩增子，而在雄性植株中不存在。引物OPW - 03产生了雄性和雌雄同株植物特异的扩增子，而在雌性植株中不存在。
