在异源真核和原核系统中表达的爱泼斯坦-巴尔病毒编码胸苷激酶的特性分析
Characterization of the Epstein-Barr virus-encoded thymidine kinase expressed in heterologous eucaryotic and procaryotic systems.
作者信息
Littler E, Arrand J R
机构信息
Department of Molecular Biology, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Withington, Manchester, United Kingdom.
出版信息
J Virol. 1988 Oct;62(10):3892-5. doi: 10.1128/JVI.62.10.3892-3895.1988.
Abstract
The establishment of mammalian and procaryotic systems which express the Epstein-Barr virus (EBV) thymidine kinase (TK) has been reported previously (E. Littler, J. Zeuthen, A. A. McBride, E. Trøst-Sørensen, K. L. Powell, J. E. Walsh-Arrand, and J. R. Arrand, EMBO J. 5:1959-1966, 1986). The EBV TK activity expressed in both of these systems was characterized by in vitro assays and found to resemble that of the herpes simplex virus TK both in its broad range of nucleoside and nucleotide utilization and also in its ability to accept antiviral nucleoside analogs as substrates. Further results are presented which suggest that these in vitro systems may prove suitable for studying the potential anti-EBV activity of other candidate antiviral compounds.
摘要
此前已有报道建立了表达爱泼斯坦-巴尔病毒（EBV）胸苷激酶（TK）的哺乳动物和原核系统（E. 利特勒、J. 措滕、A. A. 麦克布赖德、E. 特罗斯特-索伦森、K. L. 鲍威尔、J. E. 沃尔什-阿伦德和J. R. 阿伦德，《欧洲分子生物学组织杂志》5:1959 - 1966，1986年）。在这两种系统中表达的EBV TK活性通过体外测定进行了表征，发现其在核苷和核苷酸利用的广泛范围以及接受抗病毒核苷类似物作为底物的能力方面都类似于单纯疱疹病毒TK。本文还给出了进一步结果，表明这些体外系统可能证明适用于研究其他候选抗病毒化合物的潜在抗EBV活性。
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