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通过电子显微镜测定BAL 31 RNA聚合酶与PM2 DNA的结合以及对限制性内切核酸酶切割的保护作用。

Binding of BAL 31 RNA polymerase to PM2 DNA as determined by electron microscopy and protection against restriction endonuclease cleavage.

作者信息

Bull P, Susaeta M, González B, Yudelevich A

机构信息

Laboratorio de Bioquímica, Pontificia Universidad Católica de Chile, Santiago.

出版信息

J Virol. 1988 Oct;62(10):3911-3. doi: 10.1128/JVI.62.10.3911-3913.1988.

DOI:10.1128/JVI.62.10.3911-3913.1988
PMID:2843687
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC253545/
Abstract

Specific binding sites of BAL 31 RNA polymerase on PM2 DNA have been mapped by protection against HincII and HindIII cleavage and by observation of enzyme-DNA complexes by electron microscopy. Nine specific binding sites were observed at map units 0.19, 0.20, 0.28, 0.54, 0.63, 0.65, 0.71, 0.72, and 0.75 by the first method. All these sites were confirmed by electron microscopy which, in addition, revealed another site at 0.05 map unit. Published nucleotide sequences of the region surrounding sites at 0.71 and 0.75 map units show the presence of consensus sequences for procaryotic promoters.

摘要

通过防止HincII和HindIII切割以及通过电子显微镜观察酶 - DNA复合物，已绘制出BAL 31 RNA聚合酶在PM2 DNA上的特异性结合位点。通过第一种方法，在图谱单位0.19、0.20、0.28、0.54、0.63、0.65、0.71、0.72和0.75处观察到9个特异性结合位点。所有这些位点均通过电子显微镜得到证实，此外，电子显微镜还揭示了在图谱单位0.05处的另一个位点。已发表的在图谱单位0.71和0.75处位点周围区域的核苷酸序列显示存在原核启动子的共有序列。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0946/253545/e0d0842d60f3/jvirol00089-0381-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0946/253545/6a9492cf60e9/jvirol00089-0380-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0946/253545/e0d0842d60f3/jvirol00089-0381-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0946/253545/6a9492cf60e9/jvirol00089-0380-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0946/253545/e0d0842d60f3/jvirol00089-0381-a.jpg

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本文引用的文献

1
Compilation and analysis of Escherichia coli promoter DNA sequences.大肠杆菌启动子DNA序列的汇编与分析
Nucleic Acids Res. 1983 Apr 25;11(8):2237-55. doi: 10.1093/nar/11.8.2237.
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Molecular cloning and physical map of bacteriophage PM2 DNA.噬菌体PM2 DNA的分子克隆与物理图谱
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Mung bean nuclease cleavage of a dA + dT-rich sequence or an inverted repeat sequence in supercoiled PM2 DNA depends on ionic environment.绿豆核酸酶对超螺旋PM2 DNA中富含dA + dT的序列或反向重复序列的切割取决于离子环境。
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Studies on the binding of RNA polymerase to polynucleotides.RNA聚合酶与多核苷酸结合的研究。
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Properties of bacteriophage PM2: a lipid-containing bacterial virus.噬菌体PM2的特性：一种含脂质的细菌病毒。
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DNA of bacteriophage PM2: a closed circular double-stranded molecule.噬菌体PM2的DNA：一种封闭的环状双链分子。
Proc Natl Acad Sci U S A. 1969 Aug;63(4):1164-8. doi: 10.1073/pnas.63.4.1164.
8
The degree of unwinding of the DNA helix by ethidium. I. Titration of twisted PM2 DNA molecules in alkaline cesium chloride density gradients.溴化乙锭对DNA螺旋的解旋程度。I. 在碱性氯化铯密度梯度中对扭曲的PM2 DNA分子进行滴定。
J Mol Biol. 1974 Nov 15;89(4):783-801. doi: 10.1016/0022-2836(74)90053-9.
9
Class of promotor sites for Escherichia coli DNA-dependent RNA polymerase.大肠杆菌DNA依赖性RNA聚合酶的启动子位点类别。
Nature. 1974 May 17;249(454):217-21. doi: 10.1038/249217a0.
10
In vitro transcription initiation from three different Escherichia coli promoters. Effect of supercoiling.来自三种不同大肠杆菌启动子的体外转录起始。超螺旋的影响。
Eur J Biochem. 1985 Apr 15;148(2):293-8. doi: 10.1111/j.1432-1033.1985.tb08838.x.