Klein Nina, Guenther Enric, Mikus Paul, Stehling Michael K, Rubinsky Boris
Inter Science GmbH, Gisikon, Switzerland.
Prostata Center, Institut fur Bildgebende Diagnostik, Offenbach, Germany.
PeerJ. 2017 Apr 18;5:e3190. doi: 10.7717/peerj.3190. eCollection 2017.
Electrolytic ablation and electroporation based ablation are minimally invasive, non-thermal surgical technologies that employ electrical currents and electric fields to ablate undesirable cells in a volume of tissue. In this study, we explore the attributes of a new tissue ablation technology that simultaneously delivers a synergistic combination of electroporation and electrolysis (E2).
A new device that delivers a controlled dose of electroporation field and electrolysis currents in the form of a single exponential decay waveform (EDW) was applied to the pig liver, and the effect of various parameters on the extent of tissue ablation was examined with histology.
Histological analysis shows that E2 delivered as EDW can produce tissue ablation in volumes of clinical significance, using electrical and temporal parameters which, if used in electroporation or electrolysis separately, cannot ablate the tissue.
The E2 combination has advantages over the three basic technologies of non-thermal ablation: electrolytic ablation, electrochemical ablation (reversible electroporation with injection of drugs) and irreversible electroporation. E2 ablates clinically relevant volumes of tissue in a shorter period of time than electrolysis and electroporation, without the need to inject drugs as in reversible electroporation or use paralyzing anesthesia as in irreversible electroporation.
电解消融和基于电穿孔的消融是微创、非热的外科技术，它们利用电流和电场来消融组织区域内不需要的细胞。在本研究中，我们探索了一种新的组织消融技术的特性，该技术同时提供电穿孔和电解（E2）的协同组合。
将一种以单指数衰减波形（EDW）形式提供可控剂量电穿孔场和电解电流的新设备应用于猪肝，并通过组织学检查各种参数对组织消融范围的影响。
组织学分析表明，以EDW形式递送的E2能够利用电参数和时间参数产生具有临床意义的组织消融体积，而这些参数若单独用于电穿孔或电解则无法消融组织。
E2组合相对于非热消融的三种基本技术具有优势：电解消融、电化学消融（注射药物的可逆电穿孔）和不可逆电穿孔。与电解和电穿孔相比，E2能在更短的时间内消融具有临床相关性的组织体积，无需像可逆电穿孔那样注射药物，也无需像不可逆电穿孔那样使用麻痹性麻醉。
