用于眼部的诊断性超声辐射力脉冲弹性成像的安全性和可行性。一项初步的体内研究。

The safety and feasibility of diagnostic acoustic radiation force impulse elastography used for eyes. A preliminary in vivo study.

作者信息

Zha Li, Chen Ke Qi, Zheng Xiao Zhi, Wu Jing

机构信息

Department of Ophtalmology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan Province, P.R. China.

Department of Ultrasound, Yancheng Institute of Clinical (The First People's Hospital of Yancheng), Xuzhou Medical University, Yancheng, Jiangsu Province, P.R. China.

出版信息

Med Ultrason. 2017 Apr 22;19(2):185-189. doi: 10.11152/mu-996.

DOI:10.11152/mu-996

PMID:28440353

Abstract

AIMS

To assess the safety and feasibility of acoustic radiation force impulse (ARFI) elastography in the eyes of rats. Material and methods: One hundred and twenty-six SD rats were divided into 7 groups. Group 1 was the control group and Group 2 to Group 7 were investigated by ARFI elastography using increased powers of 5%, 10%, 20%, 50%, and 100% and repetitions of 5 or 10. The changes of ocular architecture structures and functions were examined by phthalmoscopic, histopathologic, and light reflex examination, and the expression level of IL-6, IL-8, and TNF-α was determined in days 1, 3, and 7 after ARFI elastography, respectively.

RESULTS

A satisfactory ARFI elastography was obtained in all SD rats. The shear wave velocity (SWV) values of soft tissues behind the eyeball were significantly greater than those of the eyes (1.89±0.95 vs. 0.84±0.36 m/s, p<0.05). The SWV values of eyes and the soft tissues behind the eyeball did not differ among any groups at any time-point (p>0.05). The SD rats of each group showed good light perception, pupillary light reflex, and avoidance reaction induced by the sudden bright light from the dark environment at any time. In each group, ocular architecture structures were well preserved, and with a normal expression level of IL-6, IL-8, and TNF-α (p>0.05).

CONCLUSIONS

Diagnostic ARFI elastography is a safe and feasible ultrasonic imaging mode and may potentially be applied for human eyes in its present form.

摘要

目的

评估声辐射力脉冲（ARFI）弹性成像技术在大鼠眼部应用的安全性和可行性。

材料与方法

126只SD大鼠分为7组。第1组为对照组，第2组至第7组采用ARFI弹性成像技术进行研究，分别使用5%、10%、20%、50%和100%的增加功率以及5次或10次重复。通过眼底镜检查、组织病理学检查和光反射检查来观察眼部结构和功能的变化，并分别在ARFI弹性成像后的第1天、第3天和第7天测定白细胞介素-6（IL-6）、白细胞介素-8（IL-8）和肿瘤坏死因子-α（TNF-α）的表达水平。

结果

所有SD大鼠均获得了满意的ARFI弹性成像结果。眼球后方软组织的剪切波速度（SWV）值显著高于眼球的SWV值（1.89±0.95 vs. 0.84±0.36 m/s，p<0.05）。在任何时间点，各实验组之间眼球和眼球后方软组织的SWV值均无差异（p>0.05）。每组SD大鼠在任何时候对光的感知、瞳孔对光反射以及从黑暗环境中突然强光照射引起的回避反应均良好。每组大鼠的眼部结构均保存完好，IL-6、IL-8和TNF-α的表达水平正常（p>0.05）。