Prasad K N, Sinha P K, Ramanujam M, Sakamoto A
Proc Natl Acad Sci U S A. 1979 Feb;76(2):829-32. doi: 10.1073/pnas.76.2.829.
Mouse neuroblastoma (NB) cells in culture were more sensitive to sodium L-ascorbate than were rat glioma cells by the criterion of growth inhibition (due to cell death and reduction in cell division). Sodium L-ascorbate at nonlethal concentrations potentiated the effect of 5-fluorouracil (FUra), x-irradiation, bleomycin, RO20-1724, prostaglandin E1, and sodium butyrate on NB cells but did not produce such an effect on glioma cells. Sodium L-ascorbate did not enhance the effect of vincristine, 6-thioguanine, or 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea (CCNU) except at higher drug doses and it reduced the cytotoxic effect of methotrexate and 5-(3,3-dimethyl-1-triazeno)-imidazole-4-carboxamide (DTIC) on NB cells. Sodium D-ascorbate produced effects similar to those produced by sodium L-ascorbate on NB cells. L-Ascorbic acid-2-sulfate (barium salt) affected neither the growth rate nor the effect of 5-FUra on NB cells. Glutathione, a reducing agent, was more toxic to NB cells in comparison to D- OR L-ascorbate; however, at a similar concentration it failed to potentiate the effect of 5-FUra on NB cells.
通过生长抑制标准（由于细胞死亡和细胞分裂减少），培养中的小鼠神经母细胞瘤（NB）细胞比大鼠胶质瘤细胞对L-抗坏血酸钠更敏感。非致死浓度的L-抗坏血酸钠增强了5-氟尿嘧啶（FUra）、X射线照射、博来霉素、RO20-1724、前列腺素E1和丁酸钠对NB细胞的作用，但对胶质瘤细胞没有产生这种作用。L-抗坏血酸钠除了在较高药物剂量下外，没有增强长春新碱、6-硫鸟嘌呤或1-(2-氯乙基)-3-环己基-1-亚硝基脲（CCNU）的作用，并且它降低了甲氨蝶呤和5-(3,3-二甲基-1-三氮烯)-咪唑-4-甲酰胺（DTIC）对NB细胞的细胞毒性作用。D-抗坏血酸钠对NB细胞产生的作用与L-抗坏血酸钠产生的作用相似。L-抗坏血酸-2-硫酸盐（钡盐）既不影响NB细胞的生长速率，也不影响5-FUra对NB细胞的作用。与D-或L-抗坏血酸相比，还原剂谷胱甘肽对NB细胞的毒性更大；然而，在相似浓度下，它未能增强5-FUra对NB细胞的作用。
