通过成熟和正在成熟的非洲爪蟾卵母细胞的电流。
Electrical currents through full-grown and maturing Xenopus oocytes.
作者信息
Robinson K R
出版信息
Proc Natl Acad Sci U S A. 1979 Feb;76(2):837-41. doi: 10.1073/pnas.76.2.837.
Abstract
An extracellular vibrating electrode was used to map the current pattern around Xenopus laevis oocytes. Current was found to enter the animal hemisphere and leave the vegetal hemisphere; in fully grown oocytes from which the follicle cells had been removed, the maximal current density was about 1 microamperemeter/cm2. This current decreased to nearly zero in response to progesterone and several other maturation-producing agents. In the case of progesterone, the decline began within a few minutes of the addition of the hormone and proceeded with a half-time of about 20 min. An analysis of the effects on the current of the removal or addition of various ions and drugs led to the inference that the major current-carrying ion was chloride and that the chloride permeability was controlled by calcium.
摘要
使用一个细胞外振动电极来绘制非洲爪蟾卵母细胞周围的电流模式。发现电流进入动物半球并离开植物半球；在去除了卵泡细胞的完全成熟的卵母细胞中，最大电流密度约为1微安/平方厘米。这种电流在孕酮和其他几种诱导成熟的物质作用下降至几乎为零。就孕酮而言，在添加该激素后的几分钟内就开始下降，下降半衰期约为20分钟。对去除或添加各种离子和药物对电流影响的分析得出推断，主要的载流离子是氯离子，并且氯离子通透性受钙控制。
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