一种用于测量稳定细胞外电流的超灵敏振动探针。
An ultrasensitive vibrating probe for measuring steady extracellular currents.
作者信息
Jaffe L F, Nuccitelli R
出版信息
J Cell Biol. 1974 Nov;63(2 Pt 1):614-28. doi: 10.1083/jcb.63.2.614.
Abstract
We describe a vibrating probe system for measuring relatively steady electrical current densities near individual living cells. It has a signal-to-noise ratio at least 100 times greater than previously available techniques. Thus it can be used to detect current densities as small as 10 nA/cm(2) in serum when a 30-microm diameter probe is vibrated at 200 Hz between two points 30 microm apart, and the amplifier's time constant is set at 10 s. Moreover, it should be generally insensitive to interference by concentration gradients. It has been first used to reveal and study 100-s long current pulses which developing fucoid embryos drive through themselves.
摘要
我们描述了一种用于测量单个活细胞附近相对稳定电流密度的振动探针系统。其信噪比至少比以前的技术高100倍。因此，当直径为30微米的探针在相距30微米的两点之间以200赫兹振动，且放大器的时间常数设置为10秒时，该系统可用于检测血清中低至10 nA/cm²的电流密度。此外，它通常应不受浓度梯度干扰的影响。它首次被用于揭示和研究发育中的岩藻胚胎自身驱动的长达100秒的电流脉冲。
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