Lam B K, Wong P Y
Department of Pharmacology, New York Medical College, Valhalla 10595.
Adv Exp Med Biol. 1988;229:51-9. doi: 10.1007/978-1-4757-0937-7_5.
[1-14C]-Eicosapentaenoic acid (EPA) was incubated with porcine leukocytes. Three polar metabolites were isolated after RP-HPLC separation in addition to pentaene leukotrienes and mono-hydroxy fatty acids. These compounds display U.V. absorbance with U.V. lambda max at 302 nm with shoulders at 289 and 317 nm which were typical of a conjugated tetraenes. Using an alkaline RP-HPLC solvent system, it was found that these three compounds co-eluted with synthetic standards of lipoxin A5, lipoxin B5 and 5S, 6S, 15S-lipoxin A5 [6-S-LXA5] and were identified accordingly. Their structures were further confirmed by GC/MS analysis. When tested for biological activities, it was found that both lipoxin A5 and lipoxin B5 induce superoxide anion generation in canine neutrophils. Furthermore, LXA5 caused a dose-related contraction of isolated rat tail artery. The biological potency of 5-series lipoxins were similar to those of 4-series.
[1-¹⁴C]-二十碳五烯酸（EPA）与猪白细胞一起孵育。除了戊烯白三烯和单羟基脂肪酸外，经反相高效液相色谱（RP-HPLC）分离后还分离出三种极性代谢物。这些化合物在紫外光下有吸收，紫外光最大吸收波长在302nm，在289和317nm处有肩峰，这是共轭四烯的典型特征。使用碱性RP-HPLC溶剂系统，发现这三种化合物与脂氧素A5、脂氧素B5和5S，6S，15S-脂氧素A5 [6-S-LXA5]的合成标准品共洗脱，并据此进行了鉴定。它们的结构通过气相色谱/质谱（GC/MS）分析得到进一步证实。在测试生物活性时，发现脂氧素A5和脂氧素B5都能诱导犬中性粒细胞产生超氧阴离子。此外，LXA5引起离体大鼠尾动脉的剂量相关收缩。5-系列脂氧素的生物活性与4-系列相似。
