金属离子是否促进了不依赖2,3-二磷酸甘油酸的磷酸甘油酸变位酶的再激活？
Do metal ions promote the re-activation of the 2,3-bisphosphoglycerate-independent phosphoglycerate mutases?
作者信息
Johnson C M, Price N C
机构信息
Department of Biological Science, University of Stirling, Scotland, U.K.
出版信息
Biochem J. 1988 May 15;252(1):111-7. doi: 10.1042/bj2520111.
Abstract
It has been reported [Smith, McWilliams & Hass (1986) Biochem. Biophys. Res. Commun. 136, 336-340] that addition of certain metal ions, notably Co2+ and Mn2+, promoted the refolding of denatured phosphoglycerate mutase from wheat germ. We have re-investigated these experiments and have shown that, when precautions are taken to avoid artefacts in the assay system, the metal ions do not promote any re-activation of the denatured wheat-germ or Aspergillus nidulans enzymes. An alternative explanation is offered for the observations of Smith et al. (1986).
摘要
据报道[史密斯、麦克威廉姆斯和哈斯（1986年），《生物化学与生物物理研究通讯》136卷，第336 - 340页]，添加某些金属离子，特别是Co2+和Mn2+，可促进小麦胚芽中变性磷酸甘油酸变位酶的重折叠。我们重新研究了这些实验，并表明，当采取预防措施避免检测系统中出现假象时，金属离子不会促进变性小麦胚芽或构巢曲霉酶的任何重新激活。针对史密斯等人（1986年）的观察结果提出了另一种解释。
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