通过在汽巴蓝琼脂糖凝胶上进行色谱分析区分依赖辅因子和不依赖辅因子的磷酸甘油酸变位酶
Distinction between cofactor-dependent and -independent phosphoglycerate mutases by chromatography on Cibacron Blue-Sepharose.
作者信息
Price N C, Stevens E
出版信息
Biosci Rep. 1983 Sep;3(9):857-61. doi: 10.1007/BF01133784.
PMID:6315103
Abstract
The binding of phosphoglycerate mutases from a variety of sources to Cibacron Blue-Sepharose has been examined. Those enzymes which are dependent on 2,3-bisphosphoglycerate (BPG) for activity bind to the immobilized dye and can be eluted by BPG. Those enzymes which are independent of BPG do not bind to the immobilized dye. The possible structural significance of this distinction is discussed.
摘要
对来自多种来源的磷酸甘油酸变位酶与汽巴蓝琼脂糖的结合情况进行了研究。那些活性依赖于2,3-二磷酸甘油酸（BPG）的酶会与固定化染料结合，并且可以被BPG洗脱。那些不依赖BPG的酶则不会与固定化染料结合。讨论了这种差异可能具有的结构意义。
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