Cerium oxide nanoparticles (CeO NPs) improve the developmental competence of in vitro-matured prepubertal ovine oocytes.

The present study investigated whether supplementation with different doses of cerium dioxide nanoparticles (CeO NPs) during in vitro maturation (IVM) of prepubertal ovine oocytes influenced their embryonic development in vitro. Cumulus-oocyte complexes derived from the ovaries of slaughtered prepubertal sheep underwent IVM with CeONPs (0, 44, 88 or 220µg mL). Matured oocytes were fertilised in vitro and zygotes were cultured for 7 days. The results demonstrated that CeONPs were internalised in the cumulus cells and not in the oocyte. The treatment with CeONPs did not affect nuclear maturation or intracellular levels of reactive oxygen species of the oocytes. The percentage of oocytes with regular chromatin configuration and cytoskeleton structures when treated with 44µg mL CeONPs was similar to oocytes matured in the absence of CeONPs and significantly higher than those treated with 88 or 220µg mL CeONPs. The relative quantification of transcripts in the cumulus cells of oocytes matured with 44µg mL CeONPs showed a statistically lower mRNA abundance of BCL2-associated X protein (BAX), B-cell CLL/lymphoma 2 (BCL2) and superoxide dismutase 1 (SOD1) compared with the 0µg mL CeO2 NPs group. A concentration of 44µg mL CeONPs significantly increased the blastocyst yield and their total, inner cell mass and trophectoderm cell numbers, compared with the 0 and 220µg mL groups. A low concentration of CeONPs in the maturation medium enhanced in vitro embryo production of prepubertal ovine oocytes.