枯草芽孢杆菌马伯格168染色体中天然存在的四环素抗性决定簇与枯草芽孢杆菌质粒pNS1981的四环素抗性基因的核苷酸序列同源性。
Nucleotide sequence homology of the tetracycline-resistance determinant naturally maintained in Bacillus subtilis Marburg 168 chromosome and the tetracycline-resistance gene of B. subtilis plasmid pNS1981.
作者信息
Sakaguchi R, Amano H, Shishido K
机构信息
Department of Life Science, Faculty of Science, Tokyo Institute of Technology, Yokohama, Japan.
出版信息
Biochim Biophys Acta. 1988 Sep 7;950(3):441-4. doi: 10.1016/0167-4781(88)90142-x.
The nucleotide sequence (1579 bp) of tetracycline-resistance determinant and flanking regions of the cloned 5.1 kb DNA fragment from Bacillus subtilis GSY908 chromosome (Sakaguchi, R. and Shishido, K. (1988) Biochim. Biophys. Acta 949, 49-57) were determined and compared with those of the B. subtilis tetracycline-resistance plasmid pNS1981. The tetracycline-resistance structural (tet) genes of the B. subtilis GSY908 chromosome (tetBS908) and pNS1981 (tetpNS1981) were found to be highly homologous (80% identical). Both tet genes were composed of 1374 bp and 458 amino-acid residues initiating from a GTG codon preceded by a ribosome-binding site (RBS-2). Upstream from tetBS908 there exists a short open reading frame (20 amino acids) initiating from a ATG codon preceded by its own RBS (RBS-1). This leader sequence was also highly homologous to that of tetpNS1981 except for a deletion of one bp between the RBS-1 and the ATG codon.
测定了来自枯草芽孢杆菌GSY908染色体的5.1 kb克隆DNA片段的四环素抗性决定簇及其侧翼区域的核苷酸序列（1579 bp）（坂口，R.和宍戸，K.（1988年）《生物化学与生物物理学报》949，49 - 57），并与枯草芽孢杆菌四环素抗性质粒pNS1981的序列进行了比较。发现枯草芽孢杆菌GSY908染色体（tetBS908）和pNS1981（tetpNS1981）的四环素抗性结构（tet）基因高度同源（80%相同）。两个tet基因均由1374 bp和458个氨基酸残基组成，起始于一个GTG密码子，该密码子之前有一个核糖体结合位点（RBS - 2）。在tetBS908上游存在一个短的开放阅读框（20个氨基酸），起始于一个ATG密码子，该密码子之前有其自身的RBS（RBS - 1）。除了RBS - 1和ATG密码子之间缺失一个碱基对之外，该前导序列与tetpNS1981的前导序列也高度同源。
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