Shishido K, Noguchi N, Kim C, Ando T
Plasmid. 1983 Nov;10(3):224-34. doi: 10.1016/0147-619x(83)90036-7.
When Bacillus subtilis GSY908 (recE4-) (H. C. Spatz and T. A. Trautner, 1971, Mol. Gen. Genet. 113, 174-190) protoplasts were infected with Staphylococcus aureus plasmid pNS1 specifying tetracycline resistance (Tcr) (N. Noguchi et al., 1983, Gene 21, 105-112), which was modified such that it either could not replicate or did not carry a functional Tcr gene, a plasmid with a molecular weight of 3.1 X 10(6) (4.9 kb) was generated in Tcr phenotypes. This plasmid, named Tcr pNS1981, exhibited completely different restriction endonuclease cleavage patterns to pNS1 and showed only negligible sequence homology in hybridization experiments. Southern hybridization experiments revealed that pNS1981 arises by excision of a B. subtilis chromosomal DNA sequence. No sequence corresponding to pNS1 was detectable on the chromosome of pNS1981-maintaining B. subtilis. The production of pNS1981 was also observed in B. subtilis RM125 (r-Mm-Mrec+) (T. Uozumi et al., 1977, Mol. Gen. Genet. 152, 65-69.) with almost the same frequency as B. subtilis GSY908. Since the recipient B. subtilis Marburg 168 derivatives stated above are sensitive to Tc, the results indicate that information essential for Tcr is under negative regulatory control in the integrated state on the chromosome. Restriction endonuclease analysis suggested that pNS1981 is essentially the same as pBC16, formerly found in B. cereus (K. Bernhard, H. Schrempf, and W. Goebel, 1978, J. Bacteriol. 133, 897-903).
用携带四环素抗性（Tcr）的金黄色葡萄球菌质粒pNS1（N. Noguchi等人，1983年，《基因》21卷，第105 - 112页）感染枯草芽孢杆菌GSY908（recE4 - ）（H. C. Spatz和T. A. Trautner，1971年，《分子与普通遗传学》113卷，第174 - 190页）原生质体，该质粒经过改造使其不能复制或不携带功能性Tcr基因，结果在Tcr表型中产生了一种分子量为3.1×10⁶（4.9 kb）的质粒。这种名为Tcr pNS1981的质粒，其限制性内切酶切割图谱与pNS1完全不同，并且在杂交实验中仅显示出可忽略不计的序列同源性。Southern杂交实验表明，pNS1981是由枯草芽孢杆菌染色体DNA序列切除产生的。在维持pNS1981的枯草芽孢杆菌染色体上未检测到与pNS1对应的序列。在枯草芽孢杆菌RM125（r - Mm - Mrec + ）（T. Uozumi等人，1977年，《分子与普通遗传学》152卷，第65 - 69页）中也观察到了pNS1981的产生，其频率与枯草芽孢杆菌GSY908几乎相同。由于上述受体枯草芽孢杆菌马伯格168衍生物对Tc敏感，结果表明Tcr所需的信息在染色体上整合状态时受到负调控。限制性内切酶分析表明，pNS1981与先前在蜡样芽孢杆菌中发现的pBC16基本相同（K. Bernhard、H. Schrempf和W. Goebel，1978年，《细菌学杂志》133卷，第897 - 903页）。
