Bae Seong-Yeon, Sheverdin Vadim, Maeng Jeehye, Lyoo In Kyoon, Han Pyung-Lim, Lee Kyunglim
Graduate School of Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, Seoul 03760, Korea.
Ewha Brain Institute, Department of Brain and Cognitive Sciences, College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul 03760, Korea.
Exp Neurobiol. 2017 Apr;26(2):82-89. doi: 10.5607/en.2017.26.2.82. Epub 2017 Mar 7.
Translationally controlled tumor protein (TCTP) is a cytosolic protein with microtubule stabilization and calcium-binding activities. TCTP is expressed in most organs including the nervous system. However, detailed distribution and functional significance of TCTP in the brain remain unexplored. In this study, we investigated the global and subcellular distributions of TCTP in the mouse brain. Immunohistochemical analyses with anti-TCTP revealed that TCTP was widely distributed in almost all regions of the brain including the cerebral cortex, thalamus, hypothalamus, hippocampus, and amygdala, wherein it was localized in axon tracts and axon terminals. In the hippocampus, TCTP was prominently localized to axon terminals of the perforant path in the dentate gyrus, the mossy fibers in the cornu ammonis (CA)3 region, and the Schaffer collaterals in the CA1 field, but not in cell bodies of granule cells and pyramidal neurons, and in their dendritic processes. Widespread distribution of TCTP in axon tracts and axon terminals throughout the brain suggests that TCTP is likely involved in neurotransmitter release and/or maintaining synaptic structures in the brain, and that it might have a role in maintaining synaptic functions and synaptic configurations important for normal cognitive, stress and emotional functions.
翻译调控肿瘤蛋白（TCTP）是一种具有微管稳定和钙结合活性的胞质蛋白。TCTP在包括神经系统在内的大多数器官中均有表达。然而，TCTP在脑中的详细分布和功能意义仍未得到探索。在本研究中，我们调查了TCTP在小鼠脑中的整体和亚细胞分布。用抗TCTP进行的免疫组织化学分析显示，TCTP广泛分布于脑的几乎所有区域，包括大脑皮层、丘脑、下丘脑、海马体和杏仁核，在其中定位于轴突束和轴突终末。在海马体中，TCTP主要定位于齿状回中穿通通路的轴突终末、海马角（CA）3区的苔藓纤维以及CA1区的谢弗侧支，但不在颗粒细胞和锥体细胞的胞体及其树突过程中。TCTP在全脑的轴突束和轴突终末中的广泛分布表明，TCTP可能参与神经递质释放和/或维持脑中的突触结构，并且它可能在维持对正常认知、应激和情绪功能很重要的突触功能和突触构型方面发挥作用。
